Immobilized Candida antarctica lipase-catalyzed alcoholysis of cotton seed oil in a solvent-free medium

• Published in: Bioresource technology Vol. 83; no. 2; pp. 125 - 129
• Main Authors: Köse, Öznur, Tüter, Melek, Aksoy, H.Ayşe
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 01-06-2002; Elsevier Science
• Subjects: Agronomy. Soil science and plant productions; alcohols; Alcohols - metabolism; Alcoholysis; Alkyl ester; Biodiesel; Biological and medical sciences; Biotechnology; Candida - enzymology; Candida antarctica; Chromatography; Cotton seed oil; Cottonseed Oil; Energy-Generating Resources; Enzymes, Immobilized - chemistry; Esters; Fundamental and applied biological sciences. Psychology; General agronomy. Plant production; Immobilized enzyme; immobilized enzymes; Lipase - chemistry; Lipase - metabolism; methanolysis; Pseudozyma antarctica; Solvents; Temperature; Time Factors; Transesterification; Use of agricultural and forest wastes. Biomass use, bioconversion; Candida antarctica; Alcoholysis; Alkyl ester; Cotton seed oil; Transesterification; Biodiesel; Immobilized enzyme; Short chain; Enzyme; Triacylglycerol lipase; Alcohol; Enzymatic catalysis; Esterases; Biofuel; Methanolysis; Cottonseed oil; Carboxylic ester hydrolases; Fungi; Ester; Hydrolases; Fungi Imperfecti; Thallophyta
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/S0960-8524(01)00203-6

The alcoholysis (transesterification) of the refined cotton seed oil of Turkish origin with primary and secondary alcohols was investigated in the presence of an immobilized enzyme from
Candida antarctica, commercially called Novozym 435 in a solvent-free medium. The optimum conditions of the methanolysis were as follows: 30% enzyme based on oil weight; oil/alcohol molar ratio 1:4; temperature: 50 °C and reaction time: 7 h. Maximum methyl esters (ME) yield was 91.5%. At the same conditions cotton seed oil was converted with short-chain primary and secondary alcohols to its corresponding esters with conversions between 72% and 94%. Our results indicated that alcoholysis products of cotton seed oil could be used as valuable intermediates in oleochemistry.