Diesel Exhaust Particle-Exposed Human Bronchial Epithelial Cells Induce Dendritic Cell Maturation

• Published in: Journal of Immunology Vol. 176; no. 12; pp. 7431 - 7437
• Main Authors: Bleck, Bertram, Tse, Doris B, Jaspers, Ilona, Curotto de Lafaille, Maria A, Reibman, Joan
• Format: Journal Article
• Language: English
• Published: United States Am Assoc Immnol 15-06-2006
• Subjects: Bronchi - cytology; Bronchi - drug effects; Bronchi - physiology; Cell Communication - drug effects; Cell Communication - physiology; Cell Differentiation - drug effects; Cell Differentiation - physiology; Dendritic Cells - cytology; Dendritic Cells - drug effects; Dendritic Cells - physiology; Granulocyte-Macrophage Colony-Stimulating Factor - physiology; Humans; Immunophenotyping; Lymphocyte Culture Test, Mixed; Monocytes - cytology; Monocytes - drug effects; Monocytes - physiology; Particle Size; Respiratory Mucosa - cytology; Respiratory Mucosa - drug effects; Respiratory Mucosa - physiology; Solubility; Vehicle Emissions
• ISSN: 0022-1767; 1550-6606; 1365-2567
• DOI: 10.4049/jimmunol.176.12.7431

Increased exposure to air pollutants such as diesel exhaust particles (DEP) has been proposed as one mechanism to explain the rise in allergic disorders. However, the immunologic mechanisms by which DEP enhance allergic sensitization and asthma remain unclear. We hypothesized that DEP act as an adjuvant for immature dendritic cell (DC) maturation via its effect on airway epithelial cell-derived microenvironment for DC. Immature monocyte-derived DC (iMDDC) failed to undergo phenotypic (CD80, CD83, CD86) or functional (T cell activation) maturation in response to exposure to DEP (0.001-100 mug/ml). In contrast, primary cultures of human bronchial epithelial cells (HBEC) treated with DEP induced iMDDC phenotypic maturation (2.6 +/- 0.1-fold increase in CD83 expression, n = 4, p < 0.05) and functional maturation (2.6 +/- 0.2-fold increase in T cell activation, n = 4, p < 0.05). Functional maturation of iMDDC was induced by conditioned medium derived from DEP-treated HBEC, and was inhibited in cultures with DEP-treated HBEC and blocking Abs against GM-CSF, or GM-CSF-targeted small interfering RNA. These data suggest that DEP induce Ag-independent DC maturation via epithelial cell-DC interactions mediated by HBEC-derived GM-CSF. Although additional signals may be required for polarization of DC, these data suggest a novel mechanism by which environmental pollutants alter airway immune responses.