High resolution multicolor-banding: a new technique for refined FISH analysis of human chromosomes

A new multicolor-banding technique has been developed which allows the differentiation of chromosome region specific areas at the band level. This technique is based on the use of differently labeled overlapping microdissection libraries. The changing fluorescence intensity ratios along the chromoso...

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Bibliographic Details
Published in:	Cytogenetics and cell genetics Vol. 84; no. 3-4; p. 156
Main Authors:	Chudoba, I, Plesch, A, Lörch, T, Lemke, J, Claussen, U, Senger, G
Format:	Journal Article
Language:	English
Published:	Switzerland 01-01-1999
Subjects:	Chromosome Banding - methods Chromosome Deletion Chromosome Painting - methods Chromosomes, Human - genetics Chromosomes, Human, Pair 2 - genetics Chromosomes, Human, Pair 5 - genetics DNA Probes - genetics Fluorescent Dyes Genomic Library Humans Neoplasms - genetics Reproducibility of Results Sensitivity and Specificity
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Summary:	A new multicolor-banding technique has been developed which allows the differentiation of chromosome region specific areas at the band level. This technique is based on the use of differently labeled overlapping microdissection libraries. The changing fluorescence intensity ratios along the chromosomes are used to assign different pseudo-colors to specific chromosome regions. The multicolor banding of human chromosome 5 is presented as an example.
ISSN:	0301-0171
DOI:	10.1159/000015245