Resistance and susceptibility to a protozoan parasite of cattle—Gene expression differences in macrophages from different breeds of cattle

• Published in: Veterinary immunology and immunopathology Vol. 120; no. 1; pp. 20 - 30
• Main Authors: Glass, Elizabeth J., Jensen, Kirsty
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15-11-2007
• Subjects: Animals; Bos indicus; breed differences; Cattle; cattle breeds; cattle diseases; Cells, Cultured; cytokines; disease resistance; gene expression; Gene Expression Regulation - immunology; genetic polymorphism; Genetic Predisposition to Disease; Holstein; Host–pathogen interactions; immune response; immune system; immunologic factors; Macrophage; macrophages; Macrophages - metabolism; Microarray; microarray technology; molecular genetics; pathogenesis; Sahiwal; Theileria annulata; Theileriasis - genetics; theileriosis; transcription (genetics); Host–pathogen interactions; Microarray; Theileria annulata; Macrophage
• ISSN: 0165-2427; 1873-2534
• DOI: 10.1016/j.vetimm.2007.07.013

Cattle infected with the tick-borne protozoan,
Theileria annulata, usually undergo severe morbidity, and mortality ensues in a high proportion of animals. However, we have shown that a
Bos indicus breed, the Sahiwal, which originates in a
T. annulata endemic area, is more resistant to the parasite. Although Sahiwals become infected, the breed exhibits fewer clinical signs and recovers from a dose of parasite which is fatal in the Holstein
B. taurus breed. The Sahiwals have a significantly lower fever response, and lower levels of parasite than the Holsteins. One unusual feature of this disease is the production of acute phase proteins (APP), indicating that the parasite induces high systemic levels of pro-inflammatory cytokines. In the Holsteins there is prolonged production of the APP, α
1-glycoprotein, which, in contrast, is only slightly elevated in the Sahiwals. As the parasite infects macrophages (mϕ), our hypothesis is that the Sahiwals can control the excessive production of pro-inflammatory cytokines in response to infection, and that this control is expressed at the level of the mϕ.
We thus reasoned that the genes underlying the observed difference in resistance to tropical theileriosis, might be identified by investigating gene expression differences in mϕ from both breeds. It is possible that relevant polymorphisms might in themselves result in gene expression differences, so initially we targeted likely candidates. However, we detected no differences in expression of the pro-inflammatory cytokines, tumour necrosis factor-α (TNFα), interleukin-1β (IL-1β) or IL-6, in infected mϕ. As it is more likely that polymorphisms in candidate genes influence the expression of other genes involved in interrelated pathways, we undertook a more global approach. We designed a bovine mϕ specific cDNA microarray, which contains representatives of 5000 different genes expressed in mϕ, and investigated the transcriptional responses of mϕ from both breeds in response to a variety of stimuli, including infection with
T. annulata. Our results indicate that there are fundamental differences in gene expression in mϕ from both breeds in the way they respond to infection, and even in their pre-infection resting state.