Bitter Taste Receptors Expression in Human Granulosa and Cumulus Cells: New Perspectives in Female Fertility

• Published in: Cells (Basel, Switzerland) Vol. 10; no. 11; p. 3127
• Main Authors: Semplici, Bianca, Luongo, Francesca Paola, Passaponti, Sofia, Landi, Claudia, Governini, Laura, Morgante, Giuseppe, De Leo, Vincenzo, Piomboni, Paola, Luddi, Alice
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 11-11-2021; MDPI
• Subjects: Adult; Bitter taste; bitter taste receptors; cumulus cells; Cumulus Cells - metabolism; Female; female fertility; Fertility; Fertility - physiology; Gene expression; Gene Expression Profiling; Gene Expression Regulation; Gnat3 protein; granulosa cells; Granulosa Cells - metabolism; Humans; In vitro fertilization; Infertility; Isoforms; Localization; oocyte quality; Oocytes; Oral cavity; Ovaries; Ovulation; Protein Interaction Maps; Proteins; Receptor mechanisms; Receptors, G-Protein-Coupled - genetics; Receptors, G-Protein-Coupled - metabolism; Reproduction; Reproductive system; Signal Transduction; Sperm; Taste - genetics; Taste buds; Taste receptors; Thermal cycling; Transducin; United States > US; bitter taste receptors; granulosa cells; oocyte quality; female fertility; cumulus cells
• ISSN: 2073-4409; 2073-4409
• DOI: 10.3390/cells10113127

Bitter taste receptors (TAS2RS) expression is not restricted to the oral cavity and the presence of these receptors in the male reproductive system and sperm provides insights into their possible role in human reproduction. To elucidate the potential role of TAS2Rs in the female reproductive system, we investigated the expression and localization of bitter taste receptors and the components of signal transduction cascade involved in the pathway of taste receptors in somatic follicular cells obtained from women undergoing assisted reproductive techniques. We found that TAS2R genes are expressed in both cumulus (CCs) and granulosa (GCs) cells, with TAS2R14 being the most highly expressed bitter receptor subtype. Interestingly, a slight increase in the expression of TAS2R14 and TAS2R43 was shown in both GCs and CCs in young women (
< 0.05), while a negative correlation may be established between the number of oocytes collected at the pickup and the expression of TAS2R43. Regarding α-gustducin and α-transducin, two Gα subunits expressed in the taste buds on the tongue, we provide evidence for their expression in CCs and GCs, with α-gustducin showing two additional isoforms in GCs. Finally, we shed light on the possible downstream transduction pathway initiated by taste receptor activation in the female reproductive system. Our study, showing for the first time the expression of taste receptors in the somatic ovarian follicle cells, significantly extends the current knowledge of the biological role of TAS2Rs for human female fertility.