mRNA up-regulation of MHC II and pivotal pro-inflammatory genes in normal brain aging

In normal brain aging, CNS resident macrophages exhibit increased expression of major histocompatibility complex (MHC) II expression. However, the transcriptional basis for this observation has not been clarified nor have age-related alterations in pivotal pro-inflammatory genes been characterized....

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Bibliographic Details
Published in:	Neurobiology of aging Vol. 27; no. 5; pp. 717 - 722
Main Authors:	Frank, Matthew G., Barrientos, Ruth M., Biedenkapp, Joseph C., Rudy, Jerry W., Watkins, Linda R., Maier, Steven F.
Format:	Journal Article
Language:	English
Published:	United States Elsevier Inc 01-05-2006
Subjects:	Aging Aging - genetics Animals Antigens, CD - genetics Brain - growth & development DNA Primers DNA, Complementary - biosynthesis DNA, Complementary - genetics Gene Expression Regulation, Developmental - physiology Genes, MHC Class II - genetics Hippocampus Immune Inflammation - genetics Macrophage Male MHC II Microglia Neurons - physiology Pro-inflammatory Rats Rats, Inbred F344 Receptors, Immunologic - genetics Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - biosynthesis RNA, Messenger - isolation & purification Up-Regulation - physiology MHC II Immune Aging Pro-inflammatory Hippocampus Macrophage Microglia
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Summary:	In normal brain aging, CNS resident macrophages exhibit increased expression of major histocompatibility complex (MHC) II expression. However, the transcriptional basis for this observation has not been clarified nor have age-related alterations in pivotal pro-inflammatory genes been characterized. Age-related mRNA alterations in MHC II, MHC II accessory molecules and several pro-inflammatory mediators were measured in older (24 months) and younger (3 months) male F344xBN F1 rats. Real time RT-PCR was utilized to measure steady state mRNA levels in hippocampus. Older as compared to younger animals exhibited increased mRNA levels of MHC II, CD86, CIITA and IFN-γ. Furthermore, IL-10 and CD200 mRNA, molecules that down-regulate macrophage activation, was decreased in older animals. The present results indicate that normal brain aging is characterized by a shift towards a pro-inflammatory microenvironment in the CNS.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0197-4580 1558-1497
DOI:	10.1016/j.neurobiolaging.2005.03.013