nc886, a Non-Coding RNA, Is a New Biomarker and Epigenetic Mediator of Cellular Senescence in Fibroblasts

• Published in: International journal of molecular sciences Vol. 22; no. 24; p. 13673
• Main Authors: Kim, Yuna, Ji, Hyanggi, Cho, Eunae, Park, Nok-Hyun, Hwang, Kyeonghwan, Park, Wonseok, Lee, Kwang-Soo, Park, Deokhoon, Jung, Eunsun
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 20-12-2021; MDPI
• Subjects: Aging; Biomarkers; Cell cycle; Cell Proliferation; Cells, Cultured; Cellular Senescence - drug effects; Collagen; Cyclin-Dependent Kinase Inhibitor p16 - genetics; Cyclin-dependent kinase inhibitor p21; Cyclin-Dependent Kinase Inhibitor p21 - genetics; DNA damage; DNA methylation; DNA Methylation - drug effects; Down-Regulation - drug effects; Epigenesis, Genetic - drug effects; epigenetic regulation; Epigenetics; Fibroblasts; Fibroblasts - cytology; Fibroblasts - drug effects; Fibroblasts - metabolism; Gene expression; Genetic Markers - drug effects; green tea extract; Humans; INK4a protein; Keratinocytes; Kinases; MicroRNAs - genetics; nc886; Non-coding RNA; Oxidative stress; p16 Protein; Phenotypes; Plant Extracts - pharmacology; Proteins; Reactive Oxygen Species - metabolism; replicative senescence; RNA polymerase; Senescence; Tea; Tea - chemistry; Transcription factors; Tumors; fibroblasts; replicative senescence; nc886; green tea extract; epigenetic regulation
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms222413673

Functional studies of organisms and human models have revealed that epigenetic changes can significantly impact the process of aging. Non-coding RNA (ncRNA), one of epigenetic regulators, plays an important role in modifying the expression of mRNAs and their proteins. It can mediate the phenotype of cells. It has been reported that nc886 (=vtRNA2-1 or pre-miR-886), a long ncRNA, can suppress tumor formation and photo-damages of keratinocytes caused by UVB. The aim of this study was to determine the role of nc886 in replicative senescence of fibroblasts and determine whether substances capable of controlling nc886 expression could regulate cellular senescence. In replicative senescence fibroblasts, nc886 expression was decreased while methylated nc886 was increased. There were changes of senescence biomarkers including SA-β-gal activity and expression of p16INK4A and p21Waf1/Cip1 in senescent cells. These findings indicate that the decrease of nc886 associated with aging is related to cellular senescence of fibroblasts and that increasing nc886 expression has potential to suppress cellular senescence. AbsoluTea Concentrate 2.0 (ATC) increased nc886 expression and ameliorated cellular senescence of fibroblasts by inhibiting age-related biomarkers. These results indicate that nc886 has potential as a new target for anti-aging and that ATC can be a potent epigenetic anti-aging ingredient.