Loss of the nutrient sensor TAS1R3 leads to reduced bone resorption

• Published in: Journal of physiology and biochemistry Vol. 74; no. 1; pp. 3 - 8
• Main Authors: Eaton, Michael S., Weinstein, Nicholas, Newby, Jordan B., Plattes, Maggie M., Foster, Hanna E., Arthur, Jon W., Ward, Taylor D., Shively, Stephen R., Shor, Ryann, Nathan, Justin, Davis, Hannah M., Plotkin, Lilian I., Wauson, Eric M., Dewar, Brian J., Broege, Aaron, Lowery, Jonathan W.
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01-02-2018; Springer Nature B.V
• Subjects: Amino acids; Animal Physiology; Biocompatibility; Biomedical and Life Sciences; Biomedical materials; Biomedicine; Bone growth; Bone marrow; Bone mass; Bone morphogenetic protein 2; Bone remodeling; Bone resorption; Clonal deletion; Collagen (type I); Cortical bone; Differentiation; Fructose; G protein-coupled receptors; Glutamic acid; Glycine; Human Physiology; Mice; Nutrient status; Original Article; Osteoclastogenesis; Osteoclasts; Osteogenesis; Phenotypes; Procollagen; Receptors; Rodents; Signaling; Stromal cells; Sucrose; Sweet taste; Taste receptors; Taste receptor; Osteoblast; TAS1R2; Osteoclast; TAS1R1; Bone; TAS1R3
• ISSN: 1138-7548; 1877-8755
• DOI: 10.1007/s13105-017-0596-7

The taste receptor type 1 (TAS1R) family of heterotrimeric G protein-coupled receptors participates in monitoring energy and nutrient status. TAS1R member 3 (TAS1R3) is a bi-functional protein that recognizes amino acids such as L-glycine and L-glutamate or sweet molecules such as sucrose and fructose when dimerized with TAS1R member 1 (TAS1R1) or TAS1R member 2 (TAS1R2), respectively. It was recently reported that deletion of TAS1R3 expression in
Tas1R3
mutant mice leads to increased cortical bone mass but the underlying cellular mechanism leading to this phenotype remains unclear. Here, we independently corroborate the increased thickness of cortical bone in femurs of 20-week-old male
Tas1R3
mutant mice and confirm that
Tas1R3
is expressed in the bone environment.
Tas1R3
is expressed in undifferentiated bone marrow stromal cells (BMSCs) in vitro and its expression is maintained during BMP2-induced osteogenic differentiation. However, levels of the bone formation marker procollagen type I N-terminal propeptide (PINP) are unchanged in the serum of 20-week-old
Tas1R3
mutant mice as compared to controls. In contrast, levels of the bone resorption marker collagen type I C-telopeptide are reduced greater than 60% in
Tas1R3
mutant mice. Consistent with this,
Tas1R3
and its putative signaling partner
Tas1R2
are expressed in primary osteoclasts and their expression levels positively correlate with differentiation status. Collectively, these findings suggest that high bone mass in
Tas1R3
mutant mice is due to uncoupled bone remodeling with reduced osteoclast function and provide rationale for future experiments examining the cell-type-dependent role for TAS1R family members in nutrient sensing in postnatal bone remodeling.