Sterol transporters at membrane contact sites regulate TORC1 and TORC2 signaling

• Published in: The Journal of cell biology Vol. 216; no. 9; pp. 2679 - 2689
• Main Authors: Murley, Andrew, Yamada, Justin, Niles, Bradley J, Toulmay, Alexandre, Prinz, William A, Powers, Ted, Nunnari, Jodi
• Format: Journal Article
• Language: English
• Published: United States Rockefeller University Press 04-09-2017; The Rockefeller University Press
• Subjects: Antiporters - genetics; Antiporters - metabolism; Biological Transport; Carrier Proteins - genetics; Carrier Proteins - metabolism; Cellular stress response; Cytoskeletal Proteins; Endoplasmic Reticulum - enzymology; Homeostasis; Lipid rafts; Lipids; Mechanistic Target of Rapamycin Complex 2; Membrane Microdomains - enzymology; Membranes; Multiprotein Complexes - genetics; Multiprotein Complexes - metabolism; Nucleic acids; Proteins; Regulators; RNA-Binding Proteins - genetics; RNA-Binding Proteins - metabolism; Saccharomyces cerevisiae - enzymology; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae Proteins - genetics; Saccharomyces cerevisiae Proteins - metabolism; Signal Transduction; Sterols; Sterols - metabolism; Stress response; TOR Serine-Threonine Kinases - genetics; TOR Serine-Threonine Kinases - metabolism; Transcription Factors - genetics; Transcription Factors - metabolism; Vacuoles - enzymology; Yeast
• ISSN: 0021-9525; 1540-8140
• DOI: 10.1083/jcb.201610032

Membrane contact sites (MCSs) function to facilitate the formation of membrane domains composed of specialized lipids, proteins, and nucleic acids. In cells, membrane domains regulate membrane dynamics and biochemical and signaling pathways. We and others identified a highly conserved family of sterol transport proteins (Ltc/Lam) localized at diverse MCSs. In this study, we describe data indicating that the yeast family members Ltc1 and Ltc3/4 function at the vacuole and plasma membrane, respectively, to create membrane domains that partition upstream regulators of the TORC1 and TORC2 signaling pathways to coordinate cellular stress responses with sterol homeostasis.