Analyses of Specific and Total Antibody Responses of Rabbits to Four Kinds of Immunogens

• Published in: Chemical & pharmaceutical bulletin Vol. 37; no. 4; pp. 1013 - 1018
• Main Authors: KITAGAWA, Tsunehiro, TANIMORI, Hideaki, SHIBATA, Miwako, YOSHIDA, Kensei, HU, Jian-Guo
• Format: Journal Article
• Language: English
• Published: Tokyo The Pharmaceutical Society of Japan 1989; Maruzen; Japan Science and Technology Agency
• Subjects: Animals; anti-drug antibody; Antibody Formation - drug effects; Antigens - immunology; Antineoplastic agents; Biological and medical sciences; blasticidin S; enzyme immunoassay; Enzyme-Linked Immunosorbent Assay; Female; General aspects; Medical sciences; neocarzinostatin; Pharmacology. Drug treatments; rabbit antibody; Rabbits; specific antibody response; viomycin; Antineoplastic agent; Drug; Vertebrata; Immunization; Mammalia; Antibody; Animal; Rabbit; Lagomorpha; Enzyme immunoassay
• ISSN: 0009-2363; 1347-5223
• DOI: 10.1248/cpb.37.1013

As a basic study to investigate suitable conditions to immunize rabbits with drug-immunogens, two highly sensitive and accurae enzyme immunoassays (EIAs) for specific antibody to viomycin (VM) and blasticidin S (BLS) were developed using the corresponding standard antibody, the solid-phase antigens, and enzyme-labeled goat anti-rabbit immunoglobulin G (IgG) antibody as immunological reagents. The accuracy of the assay results with these newly developed EIAs was demonstrated. The new EIAs as well as two previously developed EIAs, EIA for antibody specific to neocarzinostatin (NCS) and a sandwich EIA for rabbit IgG, were applied for analyses of the changes in contents of total and specific antibodies in rabbit antisera samples collected during immunizations with four antigens. Total IgG levels increased from 7.0-9.9 mg/ml to 30-50 mg/ml in all rabbits immunized under the same immunizing schedule, despite the use of four kinds of antigens. The highest level of specific antibodies, anti-BLS, anti-VM and anti-NCS, was 0.5 mg/ml in each case.