Activation of protein kinase C attenuates the cyclic GMP responses to C-type natriuretic peptide in cultured mouse astrocytes

• Published in: FEBS letters Vol. 308; no. 3; pp. 301 - 304
• Main Authors: Yeung, V.T.F., Ho, S.K.S., Cockram, C.S., Lee, C.M., Nicholls, M.G.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 24-08-1992; Elsevier
• Subjects: activation; Animals; Astrocyte; astrocytes; Astrocytes - drug effects; Astrocytes - metabolism; Atrial Natriuretic Factor - pharmacology; Biological and medical sciences; C-type natriuretic peptide; cell culture; Cell physiology; Cells, Cultured; Cyclic GMP; Cyclic GMP - metabolism; Dose-Response Relationship, Drug; effects on; Enzyme Activation; Fundamental and applied biological sciences. Psychology; Hormonal regulation; Mice; Molecular and cellular biology; natriuretic hormone (C-type); Natriuretic Peptide, C-Type; Nerve Tissue Proteins - pharmacology; Protein kinase C; Protein Kinase C - metabolism; response; Tetradecanoylphorbol Acetate - analogs & derivatives; Tetradecanoylphorbol Acetate - pharmacology; Protein kinase C; Astrocyte; C-type natriuretic peptide; Cyclic GMP; Cell culture; Enzyme; Peptide hormone; Rodentia; 3',5'-GMP; In vitro; Signal transduction; Vertebrata; Natriuretic hormone; Mammalia; Mouse
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/0014-5793(92)81298-Z

C-type natriuretic peptide (CNP), a recently discovered natriuretic peptide, has a potent stimulatory effect on cyclic GMP (cGMP) formation in cultured mouse astrocytes. Pretreatment of astrocytes with phorbol 12-myristate 13-acetate (PMA), an activator of protein kinase C (PKC). attenuated CNP-induced cGMP responses in a dose-dependent manner, with a half-maximal inhibitory concentration of 6 nM, whereas the inactive phorbol ester analog, 4α-phorbol 12,13-didecanoate, was without effect. In the presence of staurosporine, a PKC inhibitor, the inhibitory effect of PMA on CNP-stimulated cGMP production was reversed. These results suggest that PKC is an inhibitory modulator of CNP-stimulated cGMP responses in astrocytes and that CNP may interact with neuropeptides which stimulate PKC.