Mycobacterium vaccae NCTC 11659, a Soil-Derived Bacterium with Stress Resilience Properties, Modulates the Proinflammatory Effects of LPS in Macrophages

• Published in: International journal of molecular sciences Vol. 24; no. 6; p. 5176
• Main Authors: Holbrook, Evan M, Zambrano, Cristian A, Wright, Caelan T O, Dubé, Elizabeth M, Stewart, Jessica R, Sanders, William J, Frank, Matthew G, MacDonald, Andrew S, Reber, Stefan O, Lowry, Christopher A
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 08-03-2023; MDPI
• Subjects: Anxiety disorders; Asthma; Bias; Central nervous system; Cytokines; Emotional disorders; Etiology; Gene expression; Genotype & phenotype; Humans; Hypotheses; IL-10; IL-12; IL-23; Immune system; Immunization; Immunoregulation; Inflammation; Interleukin 10; Lipopolysaccharides; Lipopolysaccharides - pharmacology; macrophage; Macrophages; Mental depression; Mental disorders; Microorganisms; monocyte; Monocytes; Mood disorders; Mycobacterium; Nervous system; Neuroinflammatory Diseases; Post traumatic stress disorder; Resilience; Risk factors; Soil bacteria; Soil microorganisms; Soil stresses; Transforming growth factor-b1; Tumor necrosis factor-TNF; Mycobacterium vaccae; THP-1; TGF-β1; immunoregulation; macrophage; monocyte; IL-12; IL-23; real-time RT-PCR; IL-10
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms24065176

Inflammatory conditions, including allergic asthma and conditions in which chronic low-grade inflammation is a risk factor, such as stress-related psychiatric disorders, are prevalent and are a significant cause of disability worldwide. Novel approaches for the prevention and treatment of these disorders are needed. One approach is the use of immunoregulatory microorganisms, such as
NCTC 11659, which have anti-inflammatory, immunoregulatory, and stress-resilience properties. However, little is known about how
NCTC 11659 affects specific immune cell targets, including monocytes, which can traffic to peripheral organs and the central nervous system and differentiate into monocyte-derived macrophages that, in turn, can drive inflammation and neuroinflammation. In this study, we investigated the effects of
NCTC 11659 and subsequent lipopolysaccharide (LPS) challenge on gene expression in human monocyte-derived macrophages. THP-1 monocytes were differentiated into macrophages, exposed to
NCTC 11659 (0, 10, 30, 100, 300 µg/mL), then, 24 h later, challenged with LPS (0, 0.5, 2.5, 250 ng/mL), and assessed for gene expression 24 h following challenge with LPS. Exposure to
NCTC 11659 prior to challenge with higher concentrations of LPS (250 ng/mL) polarized human monocyte-derived macrophages with decreased
,
, and
expression relative to
and
mRNA expression. These data identify human monocyte-derived macrophages as a direct target of
NCTC 11659 and support the development of
NCTC 11659 as a potential intervention to prevent stress-induced inflammation and neuroinflammation implicated in the etiology and pathophysiology of inflammatory conditions and stress-related psychiatric disorders.