Enteral vaccination of rats against Fasciola hepatica using recombinant cysteine proteinase (cathepsin L1)

• Published in: Vaccine Vol. 25; no. 18; pp. 3619 - 3628
• Main Authors: KESIK, Malgorzata, JEDLINA-PANASIUK, Luiza, KOZAK-CIESZCZYK, Monika, PLUCIENNICZAK, Andrzej, WEDRYCHOWICZ, Halina
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 04-05-2007; Elsevier
• Subjects: Administration, Oral; Allergy and Immunology; Animals; Applied microbiology; Biological and medical sciences; Cathepsins - genetics; Cathepsins - immunology; CD4-Positive T-Lymphocytes - immunology; CD8-Positive T-Lymphocytes - immunology; Cysteine proteinase; Escherichia coli; Escherichia coli - genetics; Fasciola hepatica; Fasciola hepatica - enzymology; Fasciola hepatica - genetics; Fasciola hepatica - immunology; Fascioliasis - immunology; Fascioliasis - prevention & control; Female; Fundamental and applied biological sciences. Psychology; Inclusion Bodies; Male; Microbiology; Oral vaccination; Peritoneal Cavity; Rats; Rats, Sprague-Dawley; Recombinant Proteins - genetics; Recombinant Proteins - immunology; Recombinant Proteins - metabolism; Trematoda; Vaccination - methods; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects); Vaccines, Synthetic - administration & dosage; Vaccines, Synthetic - immunology; Fasciola hepatica; Oral vaccination; Inclusion bodies; Cysteine proteinase; Cysteine; Rat; Enzyme; Vaccination; Rodentia; Plathelmintha; Trematoda; Peptidases; Vertebrata; Cathepsin; Mammalia; Helmintha; Hydrolases; Inclusion body; Invertebrata
• ISSN: 0264-410X; 1873-2518
• DOI: 10.1016/j.vaccine.2007.01.057

Abstract Cysteine proteinases released by Fasciola hepatica play a key role in parasite feeding, migration through host tissues and in immune evasion. Hence, a recombinant cysteine proteinase (CPFhW) expressed as inclusion bodies in Escherichia coli was used for enteral vaccination of rats against fasciolosis. We managed to activate this proteinase and found it to have cathepsin L1-like substrate preference. Enteral vaccination of rats induced a 78–80% protection against challenge with fluke metacercariae (mc). The immunised rats showed clear immunological response. The challenge with mc caused a remarkable infiltration of eosinophils into the peritoneal cavity of both the vaccinated rats and challenge control rats. However, CD8+ and CD4+ lymphocytes appeared in significantly higher numbers in the peritoneal fluid of vaccinated rats than in controls.