Allium cepa anaphase–telophase root tip chromosome aberration assay on N-methyl- N-nitrosourea, maleic hydrazide, sodium azide, and ethyl methanesulfonate

• Published in: Mutation research Vol. 390; no. 1; pp. 121 - 127
• Main Authors: Rank, J, Nielsen, M.H
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 24-04-1997
• Subjects: Allium - drug effects; Allium - genetics; ALLIUM CEPA; Anaphase - drug effects; Anaphase-telophase; Arabidopsis - drug effects; Arabidopsis - genetics; Assay; AZIDA SODICA; AZIDE DE SODIUM; Azides - toxicity; Chromosome aberration; Chromosome Aberrations; COMPOSE NUCLEIQUE; COMPUESTOS NUCLEICOS; Dose-Response Relationship, Drug; Ethyl Methanesulfonate - toxicity; GENETIC DISORDERS; Maleic Hydrazide - toxicity; Methylnitrosourea - toxicity; Micronucleus; Micronucleus Tests; Mutagenicity Tests - methods; Mutagens - toxicity; NUCLEIC COMPOUNDS; Plant genotoxicity; Plant Roots - drug effects; Plant Roots - genetics; Plants - drug effects; Plants - genetics; SODIUM AZIDE; Telophase - drug effects; TOXICIDAD; TOXICITE; TOXICITY; TRASTORNOS GENETICOS; TROUBLE GENETIQUE; Assay; Plant genotoxicity; Allium cepa; Anaphase-telophase; Chromosome aberration; Micronucleus
• ISSN: 1383-5718; 0027-5107; 1879-3592
• DOI: 10.1016/S0165-1218(97)00008-6

The Allium anaphase–telophase assay was used to show genotoxicity of
N-methyl-
N-nitrosourea (MNU), maleic hydrazide (MH), sodium azide (NaN
3) and ethyl methanesulfonate (EMS). All agents induced chromosome aberrations at statistically significant levels. The rank of the lowest doses with positive effect was as follows: NaN
3 0.3 mg/l
<
MH 1 mg/l
<
MNU 41 mg/l
<
EMS 100 mg/l. The results were compared with results from other plant assays (Arabidopsis, Vicia, Tradescantia) and for MH and MNU the values were found to be within the same range, whereas the results in the Allium test for NaN
3 and EMS were in a lower range than that found for the other plant assays. EMS and MMS (methyl methanesulfonate), two chemicals used as positive controls in mutagenicity testing, were compared in the Allium test, and MMS was found to be about ten times more potent in inducing chromosome aberrations than EMS. Recording of micronuclei in interphase cells showed that this endpoint does not give more information of clastogenicity than recording of chromosome aberrations in anaphase–telophase cells.