High-precision isotopic analysis of serum and whole blood Cu, Fe and Zn to assess possible homeostasis alterations due to bariatric surgery

• Published in: Analytical and bioanalytical chemistry Vol. 412; no. 3; pp. 727 - 738
• Main Authors: Hastuti, Agustina A. M. B., Costas-Rodríguez, Marta, Anoshkina, Yulia, Parnall, Taylor, Madura, James A., Vanhaecke, Frank
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 2020; Springer; Springer Nature B.V
• Subjects: Adult; Analysis; Analytical Chemistry; Biochemistry; Blood; Characterization and Evaluation of Materials; Chemistry; Chemistry and Materials Science; Composition; Condition monitoring; Copper; Copper - blood; Female; Food Science; Gastric Bypass; Gastrointestinal surgery; Homeostasis; Humans; Inductively coupled plasma mass spectrometry; Iron - blood; Isotope ratios; Isotopes; Isotopes - blood; Laboratory Medicine; Lipids; Mass spectrometry; Mass spectroscopy; Metals; Middle Aged; Monitoring/Environmental Analysis; Obesity; Parameters; Patients; Research Paper; Serum - chemistry; Statistical methods; Surgery; Type 2 diabetes; Weight loss; Young Adult; Zinc; Zinc - blood; Belgium; Isotopic analysis; Cu; Zn; MC-ICP-MS; Bariatric surgery; Fe
• ISSN: 1618-2642; 1618-2650
• DOI: 10.1007/s00216-019-02291-2

Bariatric surgery is an effective procedure to achieve weight loss in obese patients. However, homeostasis of essential metals may be disrupted as the main absorption site is bypassed. In this study, we determined Cu, Fe and Zn isotopic compositions in paired serum and whole blood samples of patients who underwent Roux-en-Y gastric bypass (RYGB) surgery for evaluation of longitudinal changes and their potential relation to mineral element concentrations and relevant clinical parameters used for monitoring the patient’s condition. Samples from eight patients were collected pre-surgery and at 3, 6 and 12 months post-surgery. Multi-collector inductively coupled plasma-mass spectrometry (MC-ICP-MS) was used for high-precision isotope ratio measurements. Alterations in metal homeostasis related to bariatric surgery were reflected in the serum and whole blood Cu, Fe and Zn isotopic compositions. The serum and whole blood Cu became isotopically lighter (lower δ
65
Cu values) after bariatric surgery, reaching statistical significance at 6 months post-surgery (
p
< 0.05). The difference between the serum and the whole blood Zn isotopic composition increased after surgery, reaching significance from 6 months post-surgery onwards (
p
< 0.05). Those changes in Cu, Fe and Zn isotopic compositions were not accompanied by similar changes in their respective concentrations, making isotopic analysis more sensitive to physiological changes than elemental content. Furthermore, the Zn isotopic composition correlates with blood glycaemic and lipid parameters, while the Fe isotopic composition correlates with glycaemic parameters.
Graphical Abstract