Identification and Analysis of the Expression of the PIP5K Gene Family in Tomatoes

• Published in: International journal of molecular sciences Vol. 25; no. 1; p. 159
• Main Authors: Wang, Zepeng, Wang, Zhongyu, Li, Xianguo, Chen, Zhaolong, Liu, Yuxiang, Zhang, Fulin, Dai, Qi, Yu, Qinghui, Li, Ning
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 21-12-2023; MDPI
• Subjects: Cell division; Chromosomes; Evolution; expression analysis; fruit development; Genes; Genomes; Kinases; Localization; Phylogenetics; Physiology; PIP5K gene family; Proteins; salt stress; tomato; Tomatoes; China; PIP5K gene family; fruit development; salt stress; tomato; expression analysis
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms25010159

To explore the function of phosphatidylinositol 4-phosphate 5-kinase (PIP5K) in tomatoes, members of the tomato PIP5K family were identified and characterized using bioinformatic methods, and their expression patterns were also analyzed under salt stress and in different tissues. Twenty-one PIP5K members-namely,
-
-were identified from ten chromosomes, and these were divided into three groups according to a phylogenetic analysis. Further bioinformatic analysis showed four pairs of collinear relationships and fragment replication events among the
family members. To understand the possible roles of the
s, a cis-acting element analysis was conducted, which indicated that tomato
s could be associated with plant growth, hormones, and stress responses. We further validated the results of the in silico analysis by integrating RNA-seq and qRT-PCR techniques for salt- and hormone-treated tomato plants. Our results showed that
genes exhibited tissue- and treatment-specific patterns, and some of the
s exhibited significantly altered expressions after our treatments, suggesting that they might be involved in these stresses. We selected one of the
s that responded to our treatments,
, to further understand its subcellular localization. Our results showed that
was located on the membrane. This study lays a foundation for the analysis of the biological functions of the tomato
genes and can also provide a theoretical basis for the selection and breeding of new tomato varieties and germplasm innovation, especially under salt stress.