Development of Levo-Lansoprazole Chiral Molecularly Imprinted Polymer Sensor Based on the Polylysine-Phenylalanine Complex Framework Conformational Separation

• Published in: Biosensors (Basel) Vol. 13; no. 5; p. 509
• Main Authors: Zhang, Lianming, Wang, Zian, Li, Dan, Yuan, Yali, Ouyang, Huixiang, Li, Jianping
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 28-04-2023; MDPI
• Subjects: Acetic acid; Adsorption; complex framework; Drugs; Electrochemical Techniques - methods; Electrochemistry; Electrodes; Elution; enantiomer; Enantiomers; Ethanol; Federal regulation; Fourier transforms; Gold; Imprinted polymers; Infrared spectroscopy; levo-lansoprazole; Limit of Detection; Molecular Imprinting - methods; molecularly imprinted; Molecularly Imprinted Polymers; o-Phenylenediamine; Performance evaluation; Phenylalanine; Phenylenediamine; Polylysine; Polymerization; Polymers; Polymers - chemistry; Recognition; selectivity; Self-assembly; Sensors; Ulcers; Voltammetry; United States > US; China; selectivity; enantiomer; levo-lansoprazole; molecularly imprinted; complex framework
• ISSN: 2079-6374; 2079-6374
• DOI: 10.3390/bios13050509

The efficacies and toxicities of chiral drug enantiomers are often dissimilar, necessitating chiral recognition methods. Herein, a polylysine-phenylalanine complex framework was used to prepare molecularly imprinted polymers (MIPs) as sensors with enhanced specific recognition capabilities for levo-lansoprazole. The properties of the MIP sensor were investigated using Fourier-transform infrared spectroscopy and electrochemical methods. The optimal sensor performance was achieved by applying self-assembly times of 30.0 and 25.0 min for the complex framework and levo-lansoprazole, respectively, eight electropolymerization cycles with
-phenylenediamine as the functional monomer, an elution time of 5.0 min using an ethanol/acetic acid/H
O mixture (2/3/8,
/
/
) as the eluent, and a rebound time of 10.0 min. A linear relationship was observed between the sensor response intensity (Δ
) and logarithm of the levo-lansoprazole concentration (l-g
) in the range of 1.0 × 10
-3.0 × 10
mol/L. Compared with a conventional MIP sensor, the proposed sensor showed more efficient enantiomeric recognition, with high selectivity and specificity for levo-lansoprazole. The sensor was successfully applied to levo-lansoprazole detection in enteric-coated lansoprazole tablets, thus demonstrating its suitability for practical applications.