Genome-Wide Characterization and Expression Analyses of Pleurotus ostreatus MYB Transcription Factors during Developmental Stages and under Heat Stress Based on de novo Sequenced Genome

• Published in: International journal of molecular sciences Vol. 19; no. 7; p. 2052
• Main Authors: Wang, Lining, Gao, Wei, Wu, Xiangli, Zhao, Mengran, Qu, Jibin, Huang, Chenyang, Zhang, Jinxia
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 14-07-2018; MDPI
• Subjects: Bionano; de novo; development; Fruit bodies; Functional analysis; Fungi; Gene expression; Gene mapping; Genes; Genomes; Heat; Heat stress; Introns; Mushrooms; MYB; NGS; Nucleotide sequence; Open reading frames; Phylogeny; Pleurotus ostreatus; Transcription factors; heat stress; development; Pleurotus ostreatus; de novo; MYB; Bionano; NGS
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms19072052

is a commercially grown mushroom species in China. However, studies on the mechanisms of the fruiting body development and stress response of
are still at a primary stage. In this study, we report the entire genome sequence of
CCMSSC03989. Then, we performed comprehensive genome-wide characterization and expression analysis of the MYB transcription factor family during a series of developmental stages and under the condition of heat stress. A 34.76 Mb genome was obtained through next-generation sequencing (NGS) and Bionano optical mapping approaches. The genome has a scaffold N50 of 1.1 Mb and contains 10.11% repeats, and 10,936 gene models were predicted. A total of 20
genes (
) were identified across the genome, and the full-length open reading frames were isolated. The
were classified into 1 repeat (1R), 2R, and 3R-MYB groups according to their MYB domain repeat numbers, and 3R-MYBs possessed relatively more introns than 1R and 2R-MYBs. Based on phylogenetic analysis, the
were divided into four groups and showed close relationships with the
genes of plants and fungi. RNA-sequencing (RNA-Seq) and quantitative PCR (qPCR) analyses revealed that
expression showed stage-specific patterns in reproductive stages and could be induced by heat stress. The
draft genome will promote genome-wide analysis, and our study of
will promote further functional analysis of
genes in mushrooms.