Application of preparative high-speed counter-current chromatography for the separation of flavonoids from the leaves of Byrsonima crassa Niedenzu (IK)

The methanolic extract of the leaves of the medicinal plant Byrsonima crassa (Malpighiaceae) contain flavonoids with antioxidant activity. They were separated in a preparative scale using high-speed counter-current chromatography. The optimum solvent system used was composed of a mixture of ethyl ac...

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Bibliographic Details
Published in:	Journal of Chromatography A Vol. 1035; no. 1; pp. 47 - 51
Main Authors:	Sannomiya, Miriam, Rodrigues, Clenilson M, Coelho, Roberta G, dos Santos, Lourdes C, Hiruma-Lima, Clélia A, Souza Brito, Alba R.M, Vilegas, Wagner
Format:	Journal Article
Language:	English
Published:	Amsterdam Elsevier B.V 30-04-2004 Elsevier
Subjects:	Amentoflavone Analysis Analytical biochemistry: general aspects, technics, instrumentation Analytical chemistry Analytical, structural and metabolic biochemistry Biological and medical sciences Byrsonima crassa Chemistry Chromatographic methods and physical methods associated with chromatography Counter-current chromatography Countercurrent Distribution - methods Exact sciences and technology Flavonoids Flavonoids - isolation & purification Fundamental and applied biological sciences. Psychology General pharmacology Malpighiaceae - chemistry Medical sciences Pharmacology. Drug treatments Plant Leaves - chemistry Plant materials Spectrophotometry, Ultraviolet Amentoflavone Byrsonima crassa Plant materials Flavonoids Counter-current chromatography Countercurrent chromatography Plant leaf Flavonoid Preparative chromatography Plant origin
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Summary:	The methanolic extract of the leaves of the medicinal plant Byrsonima crassa (Malpighiaceae) contain flavonoids with antioxidant activity. They were separated in a preparative scale using high-speed counter-current chromatography. The optimum solvent system used was composed of a mixture of ethyl acetate– n-propanol–water (140:8:80 (v/v/v)) and led to a successful separation between monoglucosilated flavonoids (quercetin-3- O-α- l-arabinoside, quercetin-3- O-β- d-galactoside) and the biflavonoid amentoflavone in only 3.5 h. The purities of quercetin-3- O-α- l-arabinoside (95 mg), quercetin-3- O-β- d-galactoside (16 mg) and the biflavonoid amentoflavone (114 mg) were all isolated at purity over 95%. Identification was performed by 1H NMR, 13C NMR and UV analyses.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0021-9673
DOI:	10.1016/j.chroma.2004.02.050