Photodynamic Activity of Tribenzoporphyrazines with Bulky Periphery against Wound Bacteria

• Published in: International journal of molecular sciences Vol. 21; no. 17; p. 6145
• Main Authors: Stolarska, Magdalena, Glowacka-Sobotta, Arleta, Mlynarczyk, Dariusz T, Dlugaszewska, Jolanta, Goslinski, Tomasz, Mielcarek, Jadwiga, Sobotta, Lukasz
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 26-08-2020; MDPI
• Subjects: Animals; Anti-Bacterial Agents - chemical synthesis; Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - pharmacology; Antimicrobial agents; Bacteria; Drug resistance; Emission analysis; Evaluation; Fluorescence; Humans; Infections; Macrocyclic Compounds - chemical synthesis; Macrocyclic Compounds - chemistry; Macrocyclic Compounds - pharmacology; Magnesium; Methicillin; Microbial Sensitivity Tests; Microorganisms; Molecular Structure; Nanoparticles; Neutrophils; Optical properties; Pathogens; Photobleaching; photodynamic antimicrobial therapy; Photodynamic therapy; Photosensitizing Agents - chemical synthesis; Photosensitizing Agents - chemistry; Photosensitizing Agents - pharmacology; porphyrazins; Porphyrins - chemistry; Quantum Dots; Singlet oxygen; Singlet Oxygen - metabolism; Staphylococcus aureus; Staphylococcus aureus - drug effects; Staphylococcus epidermidis - drug effects; Streptococcus pyogenes - drug effects; Sulfur; Symmetry; wound; Wound healing; Wound Infection - prevention & control; Wounds; wound; porphyrazins; singlet oxygen; photodynamic antimicrobial therapy
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms21176145

Magnesium(II) tribenzoporphyrazines with phenoxybutylsulfanyl substituents were evaluated as photosensitizers in terms of their optical properties against wound bacteria. In the UV-vis spectra of analyzed tribenzoporphyrazines, typical absorption ranges were found. However, the emission properties were very weak, with fluorescence quantum yields in the range of only 0.002-0.051. What is important, they revealed moderate abilities to form singlet oxygen with the quantum yields up to 0.27. Under irradiation, the macrocycles decomposed via photobleaching mechanism with the quantum yields up to 8.64 × 10
. The photokilling potential of tribenzoporphyrazines was assessed against
,
, as well as various strains of
, including methicillin-sensitive and-resistant bacteria. Both evaluated photosensitizers revealed high photodynamic potential against studied bacteria (>3 logs).
growth was reduced by over 5.9 log, methicillin-resistant
by 5.1 log,
by over 5.7 log, and
by over 4.7 log.