Assessment of the sublethal toxicity of organochlorine pesticide endosulfan in juvenile common carp (Cyprinus carpio)

• Published in: Journal of environmental science and health. Part A, Toxic/hazardous substances & environmental engineering Vol. 47; no. 11; pp. 1652 - 1658
• Main Authors: Salvo, Lígia M, Bainy, Afonso C. D, Ventura, Eliana C, Marques, Maria R. F, Silva, José Roberto M. C, Klemz, Cláudio, Silva de Assis, Helena C
• Format: Journal Article
• Language: English
• Published: Philadelphia, PA Taylor & Francis Group 01-09-2012; Taylor & Francis; Taylor & Francis Ltd
• Subjects: adverse effects; Animals; Applied sciences; Aquatic ecosystems; aquatic environment; Biochemistry; Biomarkers; Biomarkers - metabolism; biotransformation; Carp; Carps - metabolism; Catalase; condition factor; CYP1A; Cyprinus carpio; Cytochrome P-450 CYP1A1 - metabolism; Cytochrome P-450 Enzyme System - metabolism; cytochrome P450; dimethyl sulfoxide; Endosulfan; Endosulfan - administration & dosage; Endosulfan - toxicity; Environmental extremism; Enzymes; EROD; Exact sciences and technology; fish; Fish Proteins - metabolism; Freshwater; glucose-6-phosphate 1-dehydrogenase; Glutathione; glutathione peroxidase; glutathione transferase; glutathione-disulfide reductase; Insecticides - administration & dosage; Insecticides - toxicity; Liver; Liver - drug effects; Liver - enzymology; Microsomes - drug effects; Microsomes - enzymology; Oxidative stress; Oxidative Stress - drug effects; Pesticides; Pollution; Stress concentration; Stresses; subchronic exposure; sublethal effects; superoxide dismutase; Water Pollutants, Chemical - administration & dosage; Water Pollutants, Chemical - toxicity; xenobiotics; Phosphates; Endosulfan; EROD; Toxicity; Pesticides; Biological marker; cytochrome P450; Contamination; Aquatic environment; Pollution; CYP1A; Organochlorine compounds; Biotransformation; Oxidation; Cyprinus carpio; Xenobiotic; oxidative stress
• ISSN: 1532-4117; 1093-4529; 1532-4117
• DOI: 10.1080/10934529.2012.687236

This study is aimed at evaluating the sublethal effects of endosulfan (EDS) in juvenile common carp (Cyprinus carpio). For this purpose, fish were exposed for 15 days to the technical EDS (95% pure) diluted in dimethyl sulfoxide (DMSO) 0.1% of the total volume in water solution in a semi-static system at sublethal concentration (1 μg/L). Subsequently, the liver somatic index (LSI) and factor condition (K) were determined. The total cytocrome P450 (CYP), CYP1A isoform, and the ethoxyresorufin-O-deethylase (EROD) activity were determined from the hepatic microsomal fraction as well as the activity of the oxidative stress enzyme system such as superoxide dismutase (SOD), catalase (CAT), glutathione-S-transferase (GST), glutathione peroxidase (GPX), glutathione reductase (GR), and glucose-6-phosphate dehydrogenase (G6PDH). Among the parameters assessed, EDS at the sublethal concentration in subchronic exposure caused significant changes in liver somatic indices as well as induction of the phase I biotransformation system and oxidative stress in juvenile common carp (Cyprinus carpio). Thus, it is seen that the use of biochemical biomarkers of environmental contamination in this study proved to be an extremely important tool for detecting the adverse effects of xenobiotics in the aquatic environment, even at low concentration.