The Effect of Reduced Graphene Oxide-Coated Biphasic Calcium Phosphate Bone Graft Material on Osteogenesis

• Published in: International journal of molecular sciences Vol. 18; no. 8; p. 1725
• Main Authors: Kim, Jeong-Woo, Shin, Yong Cheol, Lee, Jin-Ju, Bae, Eun-Bin, Jeon, Young-Chan, Jeong, Chang-Mo, Yun, Mi-Jung, Lee, So-Hyoun, Han, Dong-Wook, Huh, Jung-Bo
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 08-08-2017; MDPI
• Subjects: Animals; Biocompatibility; biphasic calcium phosphate; Bone grafts; Bone growth; Bone Regeneration; Bone Substitutes - chemistry; Bone surgery; Bone Transplantation; Bones; Calcium; Calcium oxide; Calcium phosphates; Cell Line; Cell Survival; Coated Materials, Biocompatible; Coating effects; Computed tomography; concentration; Electrostatic properties; Grafting; Graphene; Graphite - chemistry; Hydroxyapatites - chemistry; Knee; Lime; Male; Osteoblasts - cytology; Osteoblasts - metabolism; Osteogenesis; Oxides - chemistry; rat; Rats; reduced graphene oxide; Regeneration; Regeneration (physiology); Skin & tissue grafts; Studies; Surface charge; X-Ray Microtomography; bone regeneration; concentration; reduced graphene oxide; rat; biphasic calcium phosphate
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms18081725

This study was conducted to evaluate the effect of biphasic calcium phosphate (BCP) coated with reduced graphene oxide (rGO) as bone graft materials on bone regeneration. The rGO-coated BCP bone graft material was fabricatied by mixing rGO and BCP at various concentrations. The surface charge of rGO-coated BCP was measured to be -14.43 mV, which formed a static electrostatic interaction. Cell viabilities were significantly diminished at higher concentrations of ≥100 μg/mL. The calvarial defects of 48 rats were implanted rGO-coated BCPs at a weight ratio of 2:1000 (rGO2), 4:1000 (rGO4), and 10:1000 (rGO10), repectively. BCP was used as a control group. The micro-CT and histological analysis were performed to evaluate new bone formation at 2 and 8 weeks after surgery. The results showed that the new bone volume (mm³) was significantly higher in the experimental groups than in the control group. Histological analysis showed that new bone areas (%) were significantly higher in the rGO2 and rGO10 than in the control, and significantly higher in rGO4 than in the rGO2 and rGO10. Conclusively, the rGO-coated BCP was found to be effective on osteogenesis and the concentration of the composite was an important factor.