Biosorption of sterols from tobacco waste extract using living and dead of newly isolated fungus Aspergillus fumigatus strain LSD-1

• Published in: Bioscience, biotechnology, and biochemistry Vol. 84; no. 7; pp. 1521 - 1528
• Main Authors: Liu, Fang, Zhang, Xiaoping, Wang, Mingdao, Guo, Lele, Yang, Yingyue, Zhao, Mingqin
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 02-07-2020; Oxford University Press
• Subjects: Absorption, Physiological; Aspergillus fumigatus; Aspergillus fumigatus - metabolism; Biodegradation, Environmental; Biomass; biosorption; Cholesterol - analogs & derivatives; Cholesterol - metabolism; Ergosterol - metabolism; Hydrogen-Ion Concentration; Microscopy, Electron, Scanning; Nicotiana - chemistry; Nicotiana - microbiology; Phytosterols - metabolism; Plant Extracts - metabolism; Plant Leaves - chemistry; Plant Leaves - microbiology; Sitosterols - metabolism; Spectroscopy, Fourier Transform Infrared; Sterols; Stigmasterol - metabolism; TWE; Water Pollutants, Chemical - metabolism; Sterols; biosorption; TWE; Aspergillus fumigatus
• ISSN: 0916-8451; 1347-6947
• DOI: 10.1080/09168451.2020.1742089

Sterols are verified to be able to produce polycyclic aromatic hydrocarbons during its pyrolysis. In this study, a kind of Aspergillus fumigatus (LSD-1) was isolated from cigar leaves, and the biosorption effects on the stigmasterol, β-sitosterol, campesterol, cholesterol, and ergosterol by using living and dead biomass of LSD-1 were investigated. The results showed that both living and dead biomass could efficiently remove these sterols in aqueous solution and tobacco waste extract (TWE). Interestingly, compared with the living biomass of LSD-1, the dead biomass of LSD-1 not only kept a high adsorption efficiency but also did not produce ergosterol. Overall, dead biomass of LSD-1 was a more suitable biosorbent to sterols in TWE. Furthermore, Brunner-Emmet-Teller (BET), Fourier transformed infrared spectrometer (FTIR) and scanning electron microscope (SEM) analysis were used to explore the biosorption process of living and dead biomass and their differences, suggesting that the biosorption of sterols was a physical process.
The living and dead biomass of fungi acted as adsorbents to remove sterols, and their possible adsorption mechanisms were explored.