Differential Expression Patterns of Pleurotus ostreatus Catalase Genes during Developmental Stages and under Heat Stress

• Published in: Genes Vol. 8; no. 11; p. 335
• Main Authors: Wang, Lining, Wu, Xiangli, Gao, Wei, Zhao, Mengran, Zhang, Jinxia, Huang, Chenyang
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 21-11-2017; MDPI
• Subjects: CAT2 gene; Catalase; Developmental stages; Fungi; Gene expression; Genomes; Heat stress; Hydrogen peroxide; Mycelia; Nucleotide sequence; Pleurotus ostreatus; Spores; heat stress; development; Pleurotus ostreatus; expression analysis; catalase
• ISSN: 2073-4425; 2073-4425
• DOI: 10.3390/genes8110335

Catalases are ubiquitous hydrogen peroxide-detoxifying enzymes. They participate in fungal growth and development, such as mycelial growth and cellular differentiation, and in protecting fungi from oxidative damage under stressful conditions. To investigate the potential functions of catalases in
, we obtained two catalase genes from a draft genome sequence of
, and cloned and characterized them (
and
).
(group II) and
(group III) encoded putative peptides of 745 and 528 amino acids, respectively. Furthermore, the gene structures were variant between
and
. Further research revealed that these two catalase genes have divergent expression patterns during different developmental stages.
/Po-cat1 was at a barely detectable level in mycelia, accumulated gradually during reproductive growth, and was maximal in separated spores. But no catalase activity of Po-cat1 was detected by native-PAGE during any part of the developmental stages. In contrast, high
/Po-cat2 expression and Po-cat2 activity found in mycelia were gradually lost during reproductive growth, and at a minimal level in separated spores. In addition, these two genes responded differentially under 32 °C and 40 °C heat stresses.
was up-regulated under both temperature conditions, while
was up-regulated at 32 °C but down-regulated at 40 °C. The accumulation of catalase proteins correlated with gene expression. These results indicate that the two divergent catalases in
may play different roles during development and under heat stress.