Iron-Binding Ligands in the Southern California Current System: Mechanistic Studies

• Published in: Frontiers in Marine Science Vol. 3
• Main Authors: Bundy, Randelle M, Jiang, Mingshun, Carter, Melissa, Barbeau, Katherine A
• Format: Journal Article
• Language: English
• Published: Lausanne Frontiers Research Foundation 15-03-2016; Frontiers Media S.A
• Subjects: Analytical methods; Bacteria; Biogeochemistry; California Current; competitive ligand exchange adsorptive cathodic stripping voltammetry; Ecological research; Incubation period; Iron; iron binding organic ligands; Iron limitation; Ligands; Long Term Ecological Research; Marine; Multiple analytical windows; Oligotrophic waters; Organic matter; Particulate organic matter; Photochemicals; Photochemistry; Phytoplankton; Plankton; Profiles; Remineralization; Seawater; Short-term changes; Subsurface water; Trends; Voltammetry; Water column; Water masses; United States > US; California; INE, Pacific, California Current
• ISSN: 2296-7745; 2296-7745
• DOI: 10.3389/fmars.2016.00027

The distributions of dissolved iron and organic iron-binding ligands were examined in water column profiles and deckboard incubation experiments in the southern California Current System (sCCS) along a transition from coastal to semi-oligotrophic waters. Analysis of the iron-binding ligand pool by competitive ligand exchange-adsorptive cathodic stripping voltammetry (CLE-ACSV) using multiple analytical windows (MAWs) revealed three classes of iron-binding ligands present throughout the water column (L1-L3), whose distributions closely matched those of dissolved iron and nitrate. Despite significant biogeochemical gradients, ligand profiles were similar between stations, with surface minima in strong ligands (L1 and L2), and relatively constant concentrations of weaker ligands (L3) down to 500 m. A phytoplankton grow-out incubation, initiated from an iron-limited water mass, showed dynamic temporal cycling of iron-binding ligands. A biological iron model was able to capture the patterns of the strong ligands in the grow-out incubation relatively well with only the microbial community as a biological source. An experiment focused on remineralization of particulate organic matter showed production of both strong and weak iron-binding ligands by the heterotrophic community, supporting a mechanism for in-situ production of both strong and weak iron-binding ligands in the subsurface water column. Photochemical experiments showed a variable influence of sunlight on the degradation of natural iron-binding ligands, providing some evidence to explain differences in surface ligand concentrations between stations. Patterns in ligand distributions between profiles and in the incubation experiments were primarily related to macronutrient concentrations, suggesting microbial remineralization processes might dominate on longer time-scales over short-term changes associated with photochemistry or phytoplankton growth.