Gene polymorphisms of Interleukin-4 in allergic rhinitis and its association with clinical phenotypes

• Published in: American journal of otolaryngology Vol. 34; no. 6; pp. 676 - 681
• Main Authors: Movahedi, Masoud, MD, Amirzargar, Ali Akbar, PhD, Nasiri, Rasoul, MD, Hirbod-Mobarakeh, Armin, MD, Farhadi, Elham, MSc, Tavakol, Marzieh, MD, Behniafard, Nasrin, MD, Ansaripour, Bita, MSc, Moradi, Batoul, MSc, Zare, Ahad, MSc, Rezaei, Nima, MD, PhD
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01-11-2013; Elsevier Limited
• Subjects: Allergies; Asthma; Case-Control Studies; Disease; Eosinophils - metabolism; Female; Gene Frequency; Genes; Genotype; Haplotypes; Homozygote; Humans; Interleukin-4 - genetics; Lymphocytes; Male; Otolaryngology; Pathogenesis; Phenotype; Polymerase Chain Reaction; Polymorphism, Single Nucleotide; Receptors, Interleukin-4 - genetics; Rhinitis, Allergic, Perennial - genetics; Rhinitis, Allergic, Seasonal - genetics; Studies; Surgery
• ISSN: 0196-0709; 1532-818X
• DOI: 10.1016/j.amjoto.2013.05.002

Abstract Background Allergic rhinitis (AR) is an inflammatory disorder of the upper airway. T-helper (Th)2 cytokines seems to have major roles behind the scene of unpleasant symptoms resulted from AR. Expression of interleukin (IL)-4 and its receptor could be affected by single nucleotide polymorphisms (SNPs). This study assessed the effect of 4 genetic variants within genes of IL-4 and IL-4R in AR. Methods Allele frequencies of one IL-4R variant ( rs1801275 ) and three SNPs of IL-4 ( rs2243248 , rs2243250 , and rs2070874 ) were investigated in 98 patients with AR, compared to a group of controls, using PCR sequence-specific-primers (PCR-SSP) method. Results Homozygosity for the C allele of rs2243250 in IL-4 was significantly overrepresented in the patient group. CC genotype in rs2070874 significantly was correlated with AR. GG/CC/CC and TT/TT/TT ( rs2243248 , rs2243250 , and rs2070874 ) haplotypes in the IL-4 gene had a significant negative correlation with AR. Conclusion SNPs in IL-4 are associated with AR and could change the clinical picture of the disease in patients.