"Green" Prussian Blue Analogues as Peroxidase Mimetics for Amperometric Sensing and Biosensing

• Published in: Biosensors (Basel) Vol. 11; no. 6; p. 193
• Main Authors: Gayda, Galina Z, Demkiv, Olha M, Gurianov, Yanna, Serkiz, Roman Ya, Klepach, Halyna M, Gonchar, Mykhailo V, Nisnevitch, Marina
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 10-06-2021; MDPI
• Subjects: amperometric (bio)sensor; Amperometry; artificial enzymes; Biochemical fuel cells; Bioelectric Energy Sources; Biofuels; Biosensing Techniques; Biosensors; Chloride; Electrical measurement; Electrochemistry; Electrodes; Enzymes; Enzymes, Immobilized; Ferrocyanides - chemistry; Fruit juices; Glucose; Glucose Oxidase; Graphite; green synthesis; Heavy metals; hexacyanoferrates of transition and noble metals; Hydrogen Peroxide; Noble metals; Optical properties; Oxidoreductases; Palladium; Peroxidase; Peroxidase - analysis; peroxidase mimetic; Peroxidases; Pigments; Reagents; Reductases; Scanning electron microscopy; Selectivity; Silicon wafers; Sodium; Superstructures; United States > US; Germany; Ukraine; amperometric (bio)sensor; glucose oxidase; glucose analysis; peroxidase mimetic; artificial enzymes; green synthesis; hexacyanoferrates of transition and noble metals
• ISSN: 2079-6374; 2079-6374
• DOI: 10.3390/bios11060193

Prussian blue analogs (PBAs) are well-known artificial enzymes with peroxidase (PO)-like activity. PBAs have a high potential for applications in scientific investigations, industry, ecology and medicine. Being stable and both catalytically and electrochemically active, PBAs are promising in the construction of biosensors and biofuel cells. The "green" synthesis of PO-like PBAs using oxido-reductase flavocytochrome
is described in this study. When immobilized on graphite electrodes (GEs), the obtained green-synthesized PBAs or hexacyanoferrates (gHCFs) of transition and noble metals produced amperometric signals in response to H
O
. HCFs of copper, iron, palladium and other metals were synthesized and characterized by structure, size, catalytic properties and electro-mediator activities. The gCuHCF, as the most effective PO mimetic with a flower-like micro/nano superstructure, was used as an H
O
-sensitive platform for the development of a glucose oxidase (GO)-based biosensor. The GO/gCuHCF/GE biosensor exhibited high sensitivity (710 A M
m
), a broad linear range and good selectivity when tested on real samples of fruit juices. We propose that the gCuHCF and other gHCFs synthesized via enzymes may be used as artificial POs in amperometric oxidase-based (bio)sensors.