Increased miR-142 Levels in Plasma and Atherosclerotic Plaques from Peripheral Artery Disease Patients with Post-Surgery Cardiovascular Events

• Published in: International journal of molecular sciences Vol. 21; no. 24; p. 9600
• Main Authors: Barbalata, Teodora, Moraru, Oriana E, Stancu, Camelia S, Devaux, Yvan, Simionescu, Maya, Sima, Anca V, Niculescu, Loredan S
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 16-12-2020; MDPI
• Subjects: Adult; Aged; Aged, 80 and over; Amputation; Arteriosclerosis; Atherosclerosis; Bioinformatics; Biological activity; biomarker; Biomarkers; Biomarkers - blood; Biomarkers - metabolism; Cardiovascular disease; Cardiovascular diseases; cardiovascular event; Cholesterol; Enzymes; Female; Femoral artery; Femoral Artery - surgery; femoral artery atherosclerotic plaque; Femur; Gene expression; Heart attacks; Humans; Inflammation; Lipids; Male; Metabolism; microRNA; MicroRNAs; MicroRNAs - blood; MicroRNAs - metabolism; Middle Aged; miRNA; Peripheral Arterial Disease - blood; Peripheral Arterial Disease - metabolism; Peripheral Arterial Disease - surgery; peripheral artery disease; Plaque, Atherosclerotic - blood; Plaque, Atherosclerotic - metabolism; Plaques; Plasma; Plasma levels; Postoperative Complications - blood; Postoperative Complications - metabolism; pri-miRNA; Regression analysis; Surgery; Vascular diseases; Vascular Grafting - adverse effects; femoral artery atherosclerotic plaque; peripheral artery disease; cardiovascular event; bioinformatics; microRNA; biomarker; pri-miRNA
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms21249600

There is an intensive effort to identify biomarkers to predict cardiovascular disease evolution. We aimed to determine the potential of microRNAs to predict the appearance of cardiovascular events (CVEs) in patients with peripheral artery disease (PAD) following femoral artery bypass surgery. Forty-seven PAD patients were enrolled and divided into two groups, without CVEs (
= 35) and with CVEs (
= 12), during 1 year follow-up. Intra-surgery atherosclerotic plaques from femoral arteries were collected and the levels of miR-142, miR-223, miR-155, and miR-92a of the primary transcripts of these microRNAs (pri-miRNAs), and gene expression of
and
were determined. Results showed that, in the plaques, miR-142, miR-223, and miR-155 expression levels were significantly increased in PAD patients with CVEs compared to those without CVEs. Positive correlations between these miRNAs and their pri-miRNAs levels and the
expression were observed. In the plasma of PAD patients with CVEs compared to those without CVEs, miR-223 and miR-142 were significantly increased. The multiple linear regression analyses revealed significant associations among several plasma lipids, oxidative and inflammatory parameters, and plasma miRNAs levels. Receiver operator characteristic (ROC) analysis disclosed that plasma miR-142 levels could be an independent predictor for CVEs in PAD patients. Functional bioinformatics analyses supported the role of these miRNAs in the regulation of biological processes associated with atherosclerosis. Taken together, these data suggest that plasma levels of miR-142, miR-223, miR-155, and miR-92a can significantly predict CVEs among PAD patients with good accuracy, and that plasma levels of miR-142 can be an independent biomarker to predict post-surgery CVEs development in PAD patients.