Phenolic extract of Eugenia uniflora L. and furanone reduce biofilm formation by Serratia liquefaciens and increase its susceptibility to antimicrobials

• Published in: Biofouling (Chur, Switzerland) Vol. 36; no. 9; pp. 1031 - 1048
• Main Authors: Rodrigues, Adeline Conceição, Almeida, Felipe Alves de, André, Cleriane, Vanetti, Maria Cristina Dantas, Pinto, Uelinton Manoel, Hassimotto, Neuza Mariko Aymoto, Vieira, Érica Nascif Rufino, Andrade, Nélio José de
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 11-11-2020; Taylor & Francis Ltd
• Subjects: Ampicillin; Anti-Infective Agents; Antibiotics; Antimicrobial agents; Bacteria; Biofilms; Dairy industry; Deoxyribonucleic acid; DNA; ellagitannin; Eugenia; Eugenia uniflora; Extracellular; Fish spoilage; Kanamycin; Oranges; Phenolic compounds; Phenols; Plant Extracts - pharmacology; Polysaccharides; Polystyrene; Polystyrene resins; Psychrotrophic microorganisms; Pulp; quercetin; Saccharides; Serratia liquefaciens; sodium dichloroisocyanurate; Spoilage; Synergistic effect; sodium dichloroisocyanurate; quercetin; kanamycin; Ampicillin; ellagitannin
• ISSN: 0892-7014; 1029-2454
• DOI: 10.1080/08927014.2020.1844881

Serratia liquefaciens is a spoilage microorganism of relevance in the dairy industry because it is psychrotrophic, able to form biofilm, and produces thermoresistant proteases and lipases. Phenolic compounds and furanones have been studied as inhibitors of biofilm formation. In this study, the potential of the pulp phenolic extract of Eugenia uniflora L. orange fruits, also called pitanga, and furanone C30 on the inhibition of biofilm formation by S. liquefaciens L53 and the susceptibility to different antimicrobials were evaluated. The pulp phenolic extract of pitanga had a high total phenolic content, being mainly composed of glycosylated quercetins and ellagitannins. Sub-inhibitory concentrations of this extract and furanone reduced biofilm formation by S. liquefaciens on polystyrene and the amount of polysaccharides, proteins and extracellular DNA in the biofilms. These biofilms were also more susceptible to kanamycin. The combinations of furanone with phenolic extract of pitanga or kanamycin showed a synergistic effect with total growth inhibition of S. liquefaciens.