Strict paternal inheritance of chloroplast DNA and maternal inheritance of mitochondrial DNA in intraspecific crosses of kiwifruit

• Published in: Theoretical and applied genetics Vol. 99; no. 1/2; pp. 314 - 322
• Main Authors: Chat, J, Chalak, L, Petit, R.J
• Format: Journal Article
• Language: English
• Published: Heidelberg Springer 01-07-1999; Berlin Springer Nature B.V; Springer Verlag
• Subjects: Actinidia deliciosa; Biological and medical sciences; chloroplast DNA; Chloroplasts; Classical genetics, quantitative genetics, hybrids; Cloning; contamination; crossing; Cultivars; cytoplasmic inheritance; Fruits; Fundamental and applied biological sciences. Psychology; genetic markers; Genetics; Genetics of eukaryotes. Biological and molecular evolution; Genomes; genomics; Life Sciences; maternal effect; Mitochondria; Mitochondrial DNA; paternal effect; pollen; Polymorphism; Pteridophyta, spermatophyta; random amplified polymorphic DNA technique; restriction fragment length polymorphism; Vegetals; New Zealand; France; China; Maternal origin; Genetic marker; Chloroplast DNA; Mitochondrial DNA; Actinidiaceae; Paternal origin; Mitochondria; Fruit crop; Dicotyledones; Angiospermae; Spermatophyta; Parental origin; Actinidia deliciosa; Chloroplast; Cytoplasmic inheritance; Polymorphism; ADN CHLOROPLASTIQUE
• ISSN: 0040-5752; 1432-2242
• DOI: 10.1007/s001220051238

Previous studies have established that chloroplasts are inherited paternally in Actinidia interspecific crosses. However, fertilisation problems in interspecific crosses may affect the transmission of organelles. Six female clones, i.e. 'Abbott', 'Bruno', 'Greensill', 'Hayward', 'Jones', 'Monty', and four male clones were used to identify cpDNA polymorphisms within the cultivated kiwifruit species A. deliciosa. The restriction patterns by HpaII of a chloroplast fragment amplified by PCR with a pair of universal primers revealed a polymorphism at the intraspecific level. The inheritance of cpDNA in 143 seedlings from three intraspecific crosses in kiwifruit (Actinidia deliciosa) was studied. All offspring displayed the restriction pattern of the paternal parent, indicating that maternal inheritance of cpDNA in kiwifruit is rare at best. Strict maternal inheritance of mtDNA was confirmed in the same crosses used to investigate cpDNA transmission. Studies of cytoplasmic inheritance in the Actinidia genus represent to date the best documented report of differential organelle inheritance of cpDNA and mtDNA in angiosperms.