Lipoprotein lipase activity in skeletal muscles of the rat: effects of denervation and tenotomy

• Published in: Journal of applied physiology (1985) Vol. 90; no. 3; pp. 954 - 960
• Main Authors: Smol, E, Zernicka, E, Czarnowski, D, Langfort, J
• Format: Journal Article
• Language: English
• Published: Bethesda, MD Am Physiological Soc 01-03-2001; American Physiological Society
• Subjects: Achilles Tendon; Animals; Biological and medical sciences; Electric Stimulation; Fundamental and applied biological sciences. Psychology; Hindlimb; In Vitro Techniques; Lipids; Lipoprotein Lipase - metabolism; Male; Muscle Denervation; Muscle Fibers, Fast-Twitch - physiology; Muscle, Skeletal - enzymology; Muscle, Skeletal - innervation; Muscle, Skeletal - physiology; Muscular system; Neurology; Proteins; Rats; Rats, Wistar; Rodents; Sciatic Nerve - physiology; Skeletal system; Striated muscle. Tendons; Tendons - physiology; Triglycerides - metabolism; Vertebrates: osteoarticular system, musculoskeletal system; Rat; Enzyme; Electrical stimulus; Tenotomy; Rodentia; Esterases; Lipoprotein lipase; Striated muscle; Carboxylic ester hydrolases; Vertebrata; Mammalia; Enzymatic activity; Triacylglycerol; Hydrolases; Gastrocnemius muscle; Denervation; Response stimulus relation
• ISSN: 8750-7587; 1522-1601
• DOI: 10.1152/jappl.2001.90.3.954

1  Department of Physiology, Academy of Physical Education,
40-065 Katowice; 2  Department of Applied Physiology, Medical
Research Centre, 02-106 Warsaw; and 3  Department of
Physiology, Medical School of Bia ystok, 15-230 Bia ystok, Poland
The effects of denervation, tenotomy,
or tenotomy with simultaneous denervation on the activity of
heparin-releasable and intracellular, residual lipoprotein lipase (LPL)
and triacylglycerol (TG) content were examined in rat skeletal muscles.
An influence of muscle electrostimulation on denervated and tenotomized
muscles was also evaluated. Activity of both LPL fractions was
decreased in denervated and/or tenotomized soleus and red portion
of gastrocnemius muscles. It was accompanied by a slight elevation of
the intracellular TG content. Electrostimulation increased activities
of both fractions of LPL in red muscles from intact hindlimbs. In
stimulated denervated muscles without or with simultaneous tenotomy,
activity of two LPL fractions was also enhanced, but control values
were reached only in denervated soleus muscle. Electrical stimulation
had no pronounced effect on LPL activity in tenotomized muscles. In
conclusion, denervation and/or tenotomy decreases LPL activity in red
muscles, indicating reduction of the muscle potential to utilize
circulating TG. Electrostimulation only partly restores the diminished
LPL activity in denervated muscles, without any effect in tenotomized ones. Thus, to maintain LPL activity in resting muscle, intact innervation and tension are needed.
lipolysis; triacylglycerol