Long-Term Mesophilic Anaerobic Co-Digestion of Swine Manure with Corn Stover and Microbial Community Analysis

• Published in: Microorganisms (Basel) Vol. 8; no. 2; p. 188
• Main Authors: Wang, Haipeng, Lim, Teng Teeh, Duong, Cuong, Zhang, Wei, Xu, Congfeng, Yan, Lei, Mei, Zili, Wang, Weidong
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI 29-01-2020; MDPI AG
• Subjects: amplicon sequencing; biogas; farm waste; semi-continuous digesters; swine manure; semi-continuous digesters; farm waste; Amplicon sequencing; swine manure; biogas
• ISSN: 2076-2607; 2076-2607
• DOI: 10.3390/microorganisms8020188

Long-term anaerobic co-digestion of swine manure (SM) and corn stover (CS) was conducted using semi-continuously loaded digesters under mesophilic conditions. A preliminary test was first conducted to test the effects of loading rates, and results indicated the 3 g-VS L
d
was the optimal loading rate. Based on the preliminary results, a verification replicated test was conducted with 3 g-VS L
d
loading rate and different SM/CS ratios (1:1, 2:1 and 1:2). Results showed that a SM/CS ratio of 2/1 was optimal, based on maximum observed methane-VS
generation and carbon conversion efficiency (72.56 ± 3.40 mL g
and 40.59%, respectively). Amplicon sequencing analysis suggested that microbial diversity was increased with CS loading. Amino-acid-degrading bacteria were abundant in the treatment groups. Archaea
could enhance biogas and methane productions.