Plasma beads loaded with Candida albicans cytosolic proteins impart protection against the fungal infection in BALB/c mice

• Published in: Vaccine Vol. 30; no. 48; pp. 6851 - 6858
• Main Authors: Ahmad, Ejaj, Fatima, Munazza T, Saleemuddin, M, Owais, M
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Ltd 06-11-2012
• Subjects: Allergy and Immunology; Animals; Antibodies; Antibodies, Fungal - blood; Antigen-presenting cells; Antigen-Presenting Cells - chemistry; Antigen-Presenting Cells - immunology; antigens; Antigens, CD - analysis; Antigens, Fungal - administration & dosage; Antigens, Fungal - immunology; Candida albicans; Candida albicans - immunology; Candidiasis; Candidiasis - immunology; Candidiasis - prevention & control; CD80 antigen; CD86 antigen; Cell Proliferation; cell-mediated immunity; crosslinking; Cytosolic protein; delayed hypersensitivity; Disease Models, Animal; Drug Carriers - administration & dosage; Female; fibrin; fungi; humoral immunity; Hypersensitivity, Delayed; Immunity (cell-mediated); Immunity (humoral); Infection; interferon-gamma; Interferon-gamma - secretion; Interleukin 4; Interleukin-4 - secretion; Liposomes; Liposomes - administration & dosage; Lymphocytes T; Mice; Mice, Inbred BALB C; Nitric oxide; Plasma - metabolism; Plasma beads; Survival Analysis; survival rate; T-lymphocytes; T-Lymphocytes - immunology; Vaccine; Vaccines; Candida albicans; Cytosolic protein; Vaccine; Plasma beads; Liposomes
• ISSN: 0264-410X; 1873-2518
• DOI: 10.1016/j.vaccine.2012.09.010

Highlights ► Development of autologous clotted plasma-based beads as an antigen delivery system. ► Plasma bead entrapped Candida cytosolic proteins evoke specific immune response in the model animals. ► Liposomized Candida antigens further entrapped in the plasma beads elicit both humoral as well as cell mediated immunity. ► The antigen entrapped in the novel vehicle imparts remarkable prophylaxis against virulent C. albicans infection in Balb/c mice.