KAT: a K-mer analysis toolkit to quality control NGS datasets and genome assemblies

• Published in: Bioinformatics (Oxford, England) Vol. 33; no. 4; pp. 574 - 576
• Main Authors: Mapleson, Daniel, Garcia Accinelli, Gonzalo, Kettleborough, George, Wright, Jonathan, Clavijo, Bernardo J
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 15-02-2017
• Subjects: Applications Notes; Fraxinus - genetics; Genome, Plant; High-Throughput Nucleotide Sequencing - methods; High-Throughput Nucleotide Sequencing - standards; Quality Control; Sequence Analysis, DNA - methods; Sequence Analysis, DNA - standards; Software
• ISSN: 1367-4803; 1367-4811
• DOI: 10.1093/bioinformatics/btw663

De novo assembly of whole genome shotgun (WGS) next-generation sequencing (NGS) data benefits from high-quality input with high coverage. However, in practice, determining the quality and quantity of useful reads quickly and in a reference-free manner is not trivial. Gaining a better understanding of the WGS data, and how that data is utilized by assemblers, provides useful insights that can inform the assembly process and result in better assemblies.
We present the K-mer Analysis Toolkit (KAT): a multi-purpose software toolkit for reference-free quality control (QC) of WGS reads and de novo genome assemblies, primarily via their k-mer frequencies and GC composition. KAT enables users to assess levels of errors, bias and contamination at various stages of the assembly process. In this paper we highlight KAT's ability to provide valuable insights into assembly composition and quality of genome assemblies through pairwise comparison of k-mers present in both input reads and the assemblies.
KAT is available under the GPLv3 license at: https://github.com/TGAC/KAT .
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Supplementary data are available at Bioinformatics online.