Biofabrication of copper oxide nanoparticles using Andean blackberry (Rubus glaucus Benth.) fruit and leaf

• Published in: Journal of Saudi Chemical Society Vol. 21; no. S1; pp. S475 - S480
• Main Authors: Kumar, Brajesh, Smita, Kumari, Cumbal, Luis, Debut, Alexis, Angulo, Yolanda
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01-01-2017; Elsevier
• Subjects: Antioxidant; Biofabrication; Copper oxide nanoparticles; TEM; UV–Vis spectroscopy; Biofabrication; Antioxidant; UV–Vis spectroscopy; TEM; Copper oxide nanoparticles
• ISSN: 1319-6103
• DOI: 10.1016/j.jscs.2015.01.009

Biofabrication of copper oxide nanoparticles (CuO-NPs) of a desired size remains a significant challenge. In this report, CuO-NPs were fabricated by treating 10mM copper nitrate with Andean blackberry fruit (ABF) and leaf (ABL); and evaluated its antioxidant activity. As-prepared NPs characterization were determined by UV–visible spectrophotometry, Dynamic Light Scattering (DLS), transmission electron microscopy (TEM) with selected area electron diffraction (SAED) and X-ray diffraction (XRD) analysis. UV–visible spectroscopy showed an electronic excitonic transition at 250–255nm clearly reveals the formation of ABF and ABL CuO-NPs. DLS analysis demonstrated mean diameter of ABF CuO-NPs (43.3nm) smaller than ABL CuO-NPs (52.5nm). TEM with SAED confirmed the CuO-NPs are spherical and of partial crystalline nature. Furthermore, the antioxidant efficacy of ABF CuO-NPs showed 89.02%, 1mM whereas ABL CuO-NPs 75.92%, 1mM against 1,1-diphenyl-2-picrylhydrazyl. From the results obtained it is suggested that green CuO-NPs could be used effectively in future biomedical concerns.