The Preventive Mechanism of Anserine on Tert-Butyl Hydroperoxide-Induced Liver Injury in L-02 Cells via Regulating the Keap1-Nrf2 and JNK-Caspase-3 Signaling Pathways

• Published in: Marine drugs Vol. 21; no. 9; p. 477
• Main Authors: Chen, Ming, Luo, Jing, Ji, Hongwu, Song, Wenkui, Zhang, Di, Su, Weiming, Liu, Shucheng
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 29-08-2023; MDPI
• Subjects: Alanine; Alanine transaminase; Analysis; anrerine; Antioxidants; Apoptosis; Aspartate aminotransferase; Bcl-2 protein; Butyl hydroperoxide; Caspase-3; Causes of; Cells; Cellular signal transduction; Diabetes; DNA fragmentation; Enzymes; Extracellular fluid; Health aspects; Heme oxygenase (decyclizing); Hepatocytes; Histidine; Identification and classification; Injury prevention; Keap1-Nrf2; Kinases; L-02 cell; Liver; Liver failure; liver injury; Oxidation; Oxidative stress; Oxidoreductases; Peptides; Physiological aspects; Prevention; Proteins; Quinone oxidoreductase; Quinones; Reactive oxygen species; Signal transduction; Structure; Survival; Toxicity; Transaminases; Transcription factors; China
• ISSN: 1660-3397; 1660-3397
• DOI: 10.3390/md21090477

Anserine is a naturally occurring histidine dipeptide with significant antioxidant activities. This study aimed to investigate the preventive mechanism of anserine on tert-butyl hydroperoxide (TBHP)-induced liver damage in a normal human liver cell line (L-02 cells). The L-02 cells were pretreated with anserine (10, 20, and 40 mmol/L) and then induced with 400 μmol/L of TBHP for 4 h. The results showed that the survival rates of L-02 cells and the contents of GSH were significantly increased with the pretreatment of anserine; the activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in the extracellular fluid were sharply decreased; and the formation of reactive oxygen species (ROS), nuclear fragmentation, and apoptosis were significantly inhibited. In addition, anserine could bind to the Kelch domain of Kelch-like ECH-associated protein 1 (Keap1) with a binding force of −7.2 kcal/mol; the protein expressions of nuclear factor-erythroid 2-related factor-2 (Nrf2), quinone oxidoreductase 1 (NQO1), heme oxygenase-1 (HO-1), and Bcl-2 were upregulated by anserine in TBHP-induced L-02 cells, with the downregulation of p-JNK and caspase-3. In conclusion, anserine might alleviated liver injury in L-02 cells via regulating related proteins in the Keap1-Nrf2 and JNK-Caspase-3 signaling pathways.