Staphylococcus aureus Strain-Dependent Biofilm Formation in Bone-Like Environment

Staphylococcus aureus Strain-Dependent Biofilm Formation in Bone-Like Environment

Staphylococcus aureus species is an important threat for hospital healthcare because of frequent colonization of indwelling medical devices such as bone and joint prostheses through biofilm formations, leading to therapeutic failure. Furthermore, bacteria within biofilm are less sensitive to the hos...

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Published in:Frontiers in microbiology Vol. 12; p. 714994
Main Authors: Lamret, Fabien, Varin-Simon, Jennifer, Velard, Frédéric, Terryn, Christine, Mongaret, Céline, Colin, Marius, Gangloff, Sophie C., Reffuveille, Fany
Format: Journal Article
Language:English
Published: Frontiers Media 07-09-2021
Frontiers Media S.A
Subjects:
biofilms
bone microenvironment
Life Sciences
Microbiology
MRSA
MSSA
prosthetic joint infection
MSSA
bone microenvironment
biofilms
MRSA
prosthetic joint infection
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Summary:Staphylococcus aureus species is an important threat for hospital healthcare because of frequent colonization of indwelling medical devices such as bone and joint prostheses through biofilm formations, leading to therapeutic failure. Furthermore, bacteria within biofilm are less sensitive to the host immune system responses and to potential antibiotic treatments. We suggested that the periprosthetic bone environment is stressful for bacteria, influencing biofilm development. To provide insights into S. aureus biofilm properties of three strains [including one methicillin-resistant S. aureus (MRSA)] under this specific environment, we assessed several parameters related to bone conditions and expected to affect biofilm characteristics. We reported that the three strains harbored different behaviors in response to the lack of oxygen, casamino acids and glucose starvation, and high concentration of magnesium. Each strain presented different biofilm biomass and live adherent cells proportion, or matrix production and composition. However, the three strains shared common responses in a bone-like environment: a similar production of extracellular DNA and engagement of the SOS response. This study is a step toward a better understanding of periprosthetic joint infections and highlights targets, which could be common among S. aureus strains and for future antibiofilm strategies.
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Edited by: Enea Gino Di Domenico, Istituti Fisioterapici Ospitalieri, Scientific Institute for Research, Hospitalization and Healthcare, Italy
Reviewed by: Mukesh Kumar Yadav, Mizoram University, India; Matthias Reiger, University of Augsburg, Germany
This article was submitted to Infectious Diseases, a section of the journal Frontiers in Microbiology
ISSN:1664-302X
1664-302X
DOI:10.3389/fmicb.2021.714994