Effective dentin restorative material based on phosphate-terminated dendrimer as artificial protein

• Published in: Colloids and surfaces, B, Biointerfaces Vol. 128; pp. 304 - 314
• Main Authors: Zhang, Hui, Yang, Jiaojiao, Liang, Kunneng, Li, Jiyao, He, Libang, Yang, Xiao, Peng, Shuangjuan, Chen, Xingyu, Ding, Chunmei, Li, Jianshu
• Format: Journal Article
• Language: English
• Published: Netherlands 01-04-2015
• Subjects: Adolescent; Adult; Animals; Biocompatibility; Biomedical materials; Biomimetic Materials - chemical synthesis; Biomimetic Materials - pharmacology; Cell Survival - drug effects; Dendrimers; Dendrimers - chemical synthesis; Dendrimers - pharmacology; Dental Materials - chemical synthesis; Dental Materials - pharmacology; Dentin; Dentin - chemistry; Extracellular Matrix Proteins - chemistry; Female; Hep G2 Cells; Humans; In vitro testing; In vivo testing; In vivo tests; Male; Molar - chemistry; Molar - surgery; Phosphoproteins - chemistry; Rats; Rats, Sprague-Dawley; Sialoglycoproteins - chemistry; Surgical implants; Tooth Extraction; Human dentin; Protein analogs; Dendrimer; Hydroxyapatite; Restorative material
• ISSN: 0927-7765; 1873-4367
• DOI: 10.1016/j.colsurfb.2015.01.058

In clinic, it calls for effective and simple materials to repair etched dentin. Bioinspired by the natural mineralization process guided by noncollagenous proteins (NCPs), in this work, we synthesized the fourth generation phosphate-terminated polyamidoamine dendrimer (G4-PO3H2) by one-step modification. We used FT-IR and 1H NMR to characterize the structure of G4-PO3H2, and MTT assay to prove its biocompatibility. It was applied as the analog of dentin phosphophoryn (DPP: a type of NCPs) to repair dentin, due to its similar dimensional scale, topological architecture and peripheral functionalities to that of DPP. By the characterization of SEM and XRD, the effective regeneration of human dentin induced by G4-PO3H2 is characterized and illustrated both in vitro (artificial saliva) and in vivo (oral cavity of rats). It is noted that the thickness of the regenerated mineral layers are more than 10 μm both in vitro and in vivo. The design strategy of G4-PO3H2 may be valuable for researchers in the fields of material science, stomatology and medicine to prepare various promising restorative nano-materials for biomineralized hard tissues such as bone and teeth.