Genetic origin of homopyrones, a rare type of hybrid phenylpropanoid- and polyketide-derived yellow pigments from Aspergillus homomorphus

• Published in: Applied microbiology and biotechnology Vol. 105; no. 12; pp. 5113 - 5121
• Main Authors: Futyma, Malgorzata E., Guo, Yaojie, Hoeck, Casper, Hoof, Jakob B., Gotfredsen, Charlotte H., Mortensen, Uffe H., Larsen, Thomas O.
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01-06-2021; Springer; Springer Nature B.V
• Subjects: Aspergillus; Biomedical and Life Sciences; Biosynthesis; Biotechnology; CRISPR; Food dyes; Fungi; Gene clusters; Genetic aspects; Genomes; Genomics; Life Sciences; Malt; Metabolites; Microbial Genetics and Genomics; Microbiology; Natural products; NMR; Nuclear magnetic resonance; Organic pigments; Origin; Phenylpropanoids; Physiological aspects; Pigments; Polyketide synthase; Polyketide Synthases; Polyketides; Proteomics; Secondary metabolites; Transcriptomics; Denmark; Pigment; Biosynthesis; Homopyrone; CRISPR-Cas9; Aspergillus homomorphus
• ISSN: 0175-7598; 1432-0614
• DOI: 10.1007/s00253-021-11379-8

In recent years, there has been an increasing demand for the replacement of synthetic food colorants with naturally derived alternatives. Filamentous fungi are prolific producers of secondary metabolites including polyketide-derived pigments, many of which have not been fully characterized yet. During our ongoing investigations of black aspergilli, we noticed that
Aspergillus homomorphus
turned yellow when cultivated on malt extract agar plates. Chemical discovery guided by UV and MS led to the isolation of two novel yellow natural products, and their structures were elucidated as aromatic α-pyrones homopyrones A (
1
) and B (
2
) by HRMS and NMR. Combined investigations including retro-biosynthesis, genome mining, and gene deletions successfully linked both compounds to their related biosynthetic gene clusters. This demonstrated that homopyrones are biosynthesized by using cinnamoyl-CoA as the starter unit, followed by extension with three malonyl-CoA units, and lactonization to give the core hybrid backbone structure. The polyketide synthase AhpA includes a
C
-methylation domain, which however seems to be promiscuous since only
2
is
C
-methylated. Altogether, the homopyrones represent a rare case of hybrid phenylpropanoid- and polyketide-derived natural products in filamentous fungi.
Key points
• Homopyrones represent a rare type of fungal polyketides synthesized from cinnamic-CoA.
• CRISPR/Cas9 technology has been firstly applied in Aspergillus homomorphus.