Comparison between ROV video and Agassiz trawl methods for sampling deep water fauna of submarine canyons in the Northwestern Mediterranean Sea with observations on behavioural reactions of target species

• Published in: Deep-sea research. Part I, Oceanographic research papers Vol. 114; pp. 149 - 159
• Main Authors: Ayma, A., Aguzzi, J., Canals, M., Lastras, G., Bahamon, N., Mecho, A., Company, J.B.
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 01-08-2016; Pergamon Press Inc
• Subjects: Abundance; Agassiz trawl; Animal behavior; Animal behaviour; Aquatic life; Autonomous vehicles; Bathypterois dubius; Brackish; Canyons; Coelorinchus; Comparative analysis; Crustaceans; Deep-sea; Ecologia marina; Fauna marina; Fish; Geryon longipes; Lepidion lepidion; Marine; Marine ecology; Marine fauna; Marine geology; Mediterranean Sea; Mediterranean Sea (northwest); Mediterrània (Mar : nord-oest); Munida; Nezumia aequalis; Northwestern Mediterranean Sea; Oceanography; Pagurus; Polyacanthonotus rissoanus; Remotely operated vehicles; ROV observations; Sampling; Submarine canyon; Submarine valleys; Trawls; Valls submarines; Mediterranean Sea; MED, Western Mediterranean; MED; Deep-sea; Animal behaviour; Submarine canyon; Agassiz trawl; Northwestern Mediterranean Sea; ROV observations
• ISSN: 0967-0637; 1879-0119
• DOI: 10.1016/j.dsr.2016.05.013

In this paper we present a comparison between Remotely Operated Vehicle (ROV) and Agassiz trawling methods for sampling deep-water fauna in three submarine canyons of the Northwestern Mediterranean Sea and describe the behavioural reactions of fishes and crustacean decapods to ROV approach. 10 ROV dives, where 3583 individuals were observed and identified to species level, and 8 Agassiz trawls were carried out in a depth range of 750–1500m. As noticed in previous studies, abundances of fishes and decapod crustaceans were much higher in the ROV videos than in Agassiz trawl samples, as the latter are designed for the retrieval of benthic, less motile species in permanent contact with the bottom. In our observations fish abundance was one order of magnitude higher with ROV (4110.22ind/km2) than with Agassiz trawl (350.88ind/km2), whereas decapod crustaceans were six times more abundant in ROV videos (6362.40ind/km2) than in Agassiz samples (1364.52ind/km2).
The behaviour of highly motile fishes was analysed in terms of stationary positioning over the seafloor and avoidance or attraction to ROV approach. The most frequently occurring fish species Coelorinchus mediterraneus, Nezumia aequalis, Bathypterois dubius, Lepidion lepidion, Trachyrincuss scabrus and Polyacanthonotus rissoanus did not react to the presence of the ROV in most cases (>50%). Only B. dubius (11%), Lepidion lepidion (14.8%), P. rissoanus (41%) and T. scabrus (14.3%) reacted to ROV approach. More than 60% of less motile species, such as crustacean decapods, did not respond to ROV presence either. Only 33.3% of Geryon longipes, 36.2% of Munida spp. and 29.79% of Pagurus spp. were observed avoiding or defensively reacting to the ROV.
The comparison of results obtained with ROV and trawl sampling is of ecological relevance since ROV can report observations in areas where trawling is technically unfeasible. The lack of reaction by most fish and crustacean decapod specimens further confirms that ROV surveying is an efficient technique to assess abundance and species composition in deep-sea waters for these motile species and questions trawl-based estimations.
•A comparison between Remotely Operated Vehicle (ROV) and Agassiz trawling methods is presented.•The behaviour of deep-sea fishes and invertebrates of the Northwestern Mediterranean submarine canyons was studied by ROV imaging.•The comparison between ROV sampling efficiency and Agassiz trawling highlighted that fish captures were 20 times higher with the ROV, with a lower efficiency for epibenthic invertebrates.•Ethological observations for fishes showed minor response to the ROV presence and new insights on decapods behaviour were obtained.•ROV surveying is an efficient technique to assess abundance and species composition in deep-sea waters for these motile species and questions trawl-based estimations.