Development of a ZHER3‐Affibody‐Targeted Nano‐Vector for Gene Delivery to HER3‐Overexpressed Breast Cancer Cells

• Published in: Macromolecular bioscience Vol. 19; no. 11; pp. e1900159 - n/a
• Main Authors: Nazari, Mahboobeh, Zamani Koukhaloo, Saeideh, Mousavi, Samira, Minai‐Tehrani, Arash, Emamzadeh, Rahman, Cheraghi, Roya
• Format: Journal Article
• Language: English
• Published: Germany Wiley Subscription Services, Inc 01-11-2019
• Subjects: affibody; Arginine; biomimetic peptide; Biomimetics; Biopolymers; Breast cancer; Deoxyribonucleic acid; Diagnostic software; Diagnostic systems; DNA; Epidermal growth factor; ErbB-2 protein; gene delivery; Gene transfer; Genes; Growth factors; human epidermal growth factor receptor‐3; Immunogenicity; Nanoparticles; nano‐vector; Peptides; RALA; Receptors; Toxicity; gene delivery; RALA; human epidermal growth factor receptor-3; breast cancer; nano-vector; affibody; biomimetic peptide
• ISSN: 1616-5187; 1616-5195
• DOI: 10.1002/mabi.201900159

Despite the initial successes of gene delivery applications, they faced on several intrinsic drawbacks including toxicity and immunogenicity. Therefore, alternative gene‐delivery systems derived from recombinant peptides have emerged and is rapidly developing. Human epidermal growth factor receptor‐3 (HER3) shows high activity in tumor resistance to anti‐human epidermal growth factor receptor 2 (HER2) therapies. In this study, an affibody molecule against HER3 is conjugated to a biomimetic peptide RALA (an amphipathic and cationic peptide enriched with arginine) and the ability of the fusion vector for targeting HER3 and afterward delivering specific genes in breast cancer cells is evaluated. The results demonstrate that the biopolymeric platform, which contains an affibody‐conjugated RALA peptide, can effectively condense DNA into nanoparticles and target the overexpressed HER3 receptors in breast cancer cells and transfer specific genes. The use of such a recombinant biopolymer may pave the way for the development of sensitive and effective diagnostic and treatment tool for breast cancer.
HER3 shows high activity in tumor resistance to anti‐HER2 therapies. Herein, the ZHER3‐affibody conjugates to a biomimetic peptide RALA. The prepared biopolymer can condense DNA into nanoparticles and target HER3 receptors and transfer specific genes. The use of such a recombinant biopolymer may pave the way for the development of effective diagnostic and treatment tool for breast cancer.