Adsorption Behavior of Amino Acids on a Stainless Steel Surface

• Published in: Journal of colloid and interface science Vol. 229; no. 1; pp. 237 - 246
• Main Authors: Imamura, Koreyoshi, Mimura, Tomoya, Okamoto, Makoto, Sakiyama, Takaharu, Nakanishi, Kazuhiro
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 01-09-2000; Elsevier
• Subjects: adsorption; amino acid; Chemistry; desorption; Exact sciences and technology; Fourier transform infrared spectroscopy; General and physical chemistry; Solid-liquid interface; stainless steel surface; Surface physical chemistry; adsorption; Fourier transform infrared spectroscopy; stainless steel surface; desorption; amino acid; Glutamic acid; Liquid solid interface; Adsorption isotherm; Desorption; Metal particle; Infrared spectrometry; Arginine; Medium effect; Lysine; Fine particle; Stainless steel; Aminoacid; Metallic adsorbent; pH; Aspartic acid; Aqueous solution
• ISSN: 0021-9797; 1095-7103
• DOI: 10.1006/jcis.2000.7016

The adsorption behavior of various amino acids on a stainless steel surface was investigated at 30°C and over a pH range of 3–10. Acidic and basic amino acids except histidine adsorbed remarkably at pH 3–4 and 7–10, respectively, and showed Langmuir-type adsorption isotherms. The effects of pH and ionic strength on the adsorption isotherms were investigated to analyze the interactions between amino acids and adsorption sites on the stainless steel. Hydrophobic amino acids and glycine showed only small adsorbed amounts at all pHs tested. For the acidic and basic amino acids, reversibility of the absorption and the influence of the ionic strength on the adsorption behavior were examined. The adsorption isotherms of the derivatives of aspartic acid were also measured in order to examine the contribution of the carboxylic groups of acidic amino acids to the adsorption. Furthermore, a Fourier-transform infrared spectroscopic analysis and semiempirical molecular orbital calculation were carried out to analyze the ionization states and the configuration of the amino acids adsorbed on a stainless steel surface. These investigations suggest that the acidic and basic amino acids adsorb through two electrostatic interactions of two ionized groups in the amino acid with a stainless steel surface.