Differential thermal sensitivity between the recipient ooplasm and the donor nucleus in Holstein and Taiwan native yellow cattle

• Published in: Theriogenology Vol. 74; no. 9; pp. 1587 - 1595
• Main Authors: Shen, P.C., Lee, J.W., Cheng, W.T.K., Su, H.Y., Lee, S.N., Liu, B.T., Wang, C.H., Chen, L.R., Ju, J.C.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01-12-2010; [Oxford]: Butterworth-Heinemann; [New York]: Elsevier Science
• Subjects: Animals; blastocyst; breed differences; cattle; Cattle - embryology; Cattle - genetics; cattle breeds; cell physiology; cloning (animals); Cloning, Organism - veterinary; cumulus oophorus; embryo (animal); Embryo Culture Techniques - veterinary; embryo transfer; Embryo Transfer - veterinary; embryogenesis; Female; female fertility; heat tolerance; heat treatment; Heat-stress; Holstein; in vitro studies; Nuclear Transfer Techniques - veterinary; nuclear transplantation; oocytes; Oocytes - cytology; ooplasm; somatic cell nuclear transfer; Somatic cell nucleus transfer; somatic cells; Stress, Physiological; Taiwan Native Yellow (cattle breed); Taiwan native yellow cattle; Temperature; Thermotolerance; viability; Taiwan; Heat-stress; Holstein; Thermotolerance; Taiwan native yellow cattle; Somatic cell nucleus transfer
• ISSN: 0093-691X; 1879-3231
• DOI: 10.1016/j.theriogenology.2010.06.029

The objective of this study was to compare thermal sensitivity of recipient ooplasm and donor nucleus from Holstein and Taiwan native yellow (TY) cows. Oocytes and cumulus cells from each breed were incubated at 43 °C (heat shock) or 38.5 °C (control) for 1 h prior to nucleus transplantation. Reconstructed embryos cloned by transfer of non-heated Holstein donor cells to heat-shocked Holstein ooplasm (Ho
+–Hd
−) had a lower (P < 0.05) blastocyst rate than those cloned from non-heated Holstein ooplasm receiving heated (Ho
−–Hd
+) or non-heated (Ho
−–Hd
−) Holstein donor cells (11.3 vs. 34.3 or 36.8%). Heat-shocked donor cells from either Holstein or TY cows did not significantly affect blastocyst rates of reconstructed embryos produced from Holstein ooplasm (30.6–32.9%). In contrast, blastocyst rates of reconstructed embryos generated with heat-shocked Holstein ooplasm were lower (P < 0.05) than that with heat-shocked TY ooplasm (11.2 vs 45.2%). Without heat shock, embryos reconstructed by transferring donor cells to ooplasm of Holstein or TY cows had similar (P > 0.05) blastocyst rates (28.9–33.3%). Transplantation of reconstructed embryos (n = 30) to recipients (n = 23) resulted in three live calves, derived from embryos cloned with TY ooplasm and donor nuclei from either Holstein (n = 2) or TY cows (n = 1). In conclusion, ooplasm of TY cattle was more resistant to heat stress than that derived from Holsteins; therefore, ooplasm may be a major determinant for thermal sensitivity in bovine oocytes and embryos.