Molecular, behavioral, and performance responses of juvenile largemouth bass acclimated to an elevated carbon dioxide environment

• Published in: Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology Vol. 186; no. 3; pp. 297 - 311
• Main Authors: Dennis, Clark E., Adhikari, Shivani, Wright, Adam W., Suski, Cory D.
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01-04-2016; Springer Nature B.V
• Subjects: Acclimatization; Animal Physiology; Animals; Anthropogenic factors; Aquatic environment; Bass; Bass - physiology; Behavior; Behavior, Animal - physiology; Biochemistry; Biomedical and Life Sciences; Biomedicine; Carbon Dioxide; Climate change; Erythrocytes; Fish Proteins - genetics; Freshwater; Gene Expression Regulation; Genes, fos; HSP70 Heat-Shock Proteins - genetics; Human Physiology; Hydrocortisone - blood; Lactic Acid - blood; Life Sciences; Metabolism; Micropterus salmoides; Original Paper; Physiology; Sodium - blood; Swimming; Zoology; Hypercarbia; Invasive species; Behavior; Performance; Stress; Acclimation
• ISSN: 0174-1578; 1432-136X
• DOI: 10.1007/s00360-016-0958-4

Aquatic hypercarbia, either naturally occurring or anthropogenically induced, can have extensive impacts on aquatic environments and resident organisms. While the impact of acute hypercarbia exposure on the behavior and physiology of fishes has been well studied, relatively little work has examined the physiological impact and acclimation capacity of fishes to chronic hypercarbia. To better understand the impacts of prolonged hypercarbia exposure, largemouth bass were held at ambient CO
2
(13 mg L
−1
) and elevated CO
2
(31 mg L
−1
; ≈21,000 µatm) for 58 days. Following this acclimation period, fish were subjected to three separate, yet complementary, experiments: (1) acute hypercarbia challenge of 120 mg L
−1
CO
2
for 1 h to quantify physiological and molecular responses; (2) hypercarbia avoidance challenge to compare CO
2
agitation and avoidance responses; and (3) swim performance challenge to quantify burst swimming performance. Acclimation to 31 mg L
−1
CO
2
resulted in a significant constitutive upregulation of
c
-
fos
expression in erythrocytes, combined with significant constitutive expression of
hsp70
in both gill and erythrocytes, relative to controls. Largemouth bass acclimated to elevated CO
2
also had a reduced glucose response (relative to controls) following an acute CO
2
exposure, indicating a reduced stress response to CO
2
stressors. In addition, largemouth bass acclimated to elevated CO
2
conditions required 50 % higher CO
2
concentrations to illicit agitation behaviors and displayed prolonged burst swimming abilities in high CO
2
environments relative to controls. Together, results demonstrate that largemouth bass exposed to chronic hypercarbia may possess a physiological advantage during periods of elevated CO
2
relative to naïve fish, which may permit increased performance in hypercarbia.