Aqueous Humor Levels of Vascular Endothelial Growth Factor and Pigment Epithelium–Derived Factor in Polypoidal Choroidal Vasculopathy and Choroidal Neovascularization

• Published in: American journal of ophthalmology Vol. 141; no. 3; pp. 456 - 462
• Main Authors: Tong, Jian-Ping, Chan, Wai-Man, Liu, David T.L., Lai, Timothy Y.Y., Choy, Kwong-Wai, Pang, Chi-Pui, Lam, Dennis S.C.
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Inc 01-03-2006; Elsevier; Elsevier Limited
• Subjects: Adult; Aged; Aged, 80 and over; Angiogenesis; Aqueous Humor - metabolism; Biological and medical sciences; Cataract Extraction; Cataracts; Choroid - blood supply; Choroidal Neovascularization - etiology; Choroidal Neovascularization - metabolism; Diabetic retinopathy; Disease; Enzyme-Linked Immunosorbent Assay; Eye Proteins - metabolism; Eye surgery; Female; Humans; Humor; Macular degeneration; Macular Degeneration - complications; Male; Medical imaging; Medical sciences; Middle Aged; Miscellaneous; Myopia; Myopia, Degenerative - metabolism; Nerve Growth Factors - metabolism; Ophthalmology; Pathogenesis; Peripheral Vascular Diseases - metabolism; Photodynamic therapy; Prospective Studies; Serpins - metabolism; Vascular endothelial growth factor; Vascular Endothelial Growth Factor A - metabolism; New York; Polypoidal choroidal vasculopathy; Biological fluid; Vascular endothelium growth factor; Choroidal neovascularization; Pigment epithelium-derived factor; Aqueous humor; Ophthalmology
• ISSN: 0002-9394; 1879-1891
• DOI: 10.1016/j.ajo.2005.10.012

To determine the aqueous levels of vascular endothelial growth factor (VEGF) and pigment epithelium–derived factor (PEDF) in patients with active polypoidal choroidal vasculopathy (PCV) and choroidal neovascularization (CNV) secondary to age-related macular degeneration (AMD) and pathologic myopia.
Prospective, comparative control study.
Aqueous humors were collected from 32 eyes of 32 patients for either active PCV or CNV. Among them, 11 eyes had active and symptomatic PCV, 12 eyes had active CNV secondary to AMD, and nine eyes had active CNV of pathologic myopia. Levels of VEGF and PEDF were determined by commercially available enzyme-linked immunosorbent assay kits. A group of 10 aqueous samples from 10 patients who underwent cataract surgery without other ocular or systemic diseases comprised the controls.
VEGF concentrations in aqueous humor were markedly increased in patients with PCV, CNV of AMD, and CNV of myopia when compared with the controls (analysis of variance [ANOVA],
P < .001). VEGF levels in eyes with PCV were, however, significantly lower than those of exudative AMD (
P = .045). The PEDF levels were also significantly different among the groups (ANOVA,
P = .001), and we observed increased levels in PCV, CNV of AMD, and CNV of myopia.
VEGF and PEDF factors were coexpressed and increased with positive correlation in aqueous humor of eyes with active PCV. The different levels of both factors in eyes of PCV and AMD might suggest distinct clinical entities or different angiogenesis courses between PCV and AMD.