SCAR and CAPS mapping of CRb, a gene conferring resistance to Plasmodiophora brassicae in Chinese cabbage (Brassica rapa ssp. pekinensis)

• Published in: Theoretical and applied genetics Vol. 108; no. 8; pp. 1458 - 1465
• Main Authors: Piao, Z.Y, Deng, Y.Q, Choi, S.R, Park, Y.J, Lim, Y.P
• Format: Journal Article
• Language: English
• Published: Heidelberg Springer 01-05-2004; Berlin Springer Nature B.V
• Subjects: amplified fragment length polymorphism; Biological and medical sciences; Brassica - genetics; Brassica - microbiology; Brassica - physiology; Brassica rapa pekinensis; Brassica rapa subsp. pekinensis; Chinese cabbage; Chromosome Mapping; Chromosome Segregation; Chromosomes, Plant - genetics; Classical genetics, quantitative genetics, hybrids; cleaved amplified polymorphic sequence; clubroot disease; CRb gene; disease resistance; dominant genes; Eukaryotic Cells - physiology; Fundamental and applied biological sciences. Psychology; fungal diseases of plants; Fungi - physiology; gene segregation; Genes, Plant - genetics; Genetic markers; Genetic Markers - genetics; genetic resistance; Genetics; Genetics of eukaryotes. Biological and molecular evolution; Genomics; Methods, theories and miscellaneous; Plant Diseases - genetics; Plant Diseases - microbiology; plant pathogenic fungi; Plasmodiophora brassicae; Polymerase Chain Reaction; Pteridophyta, spermatophyta; root diseases; sequence characterized amplified region; Vegetals; Genetic mapping; Protozoa; Sequence characterized amplified region marker; Brassica rapa; Genetic marker; Scar; Resistance; Gene; Cruciferae; Dicotyledones; Plasmodiophora brassicae; Angiospermae; Chinese; Genetics; Spermatophyta; Agriculture; Mycetozoa
• ISSN: 0040-5752; 1432-2242
• DOI: 10.1007/s00122-003-1577-5

Clubroot disease, caused by Plasmodiophora brassicae Wor., is highly damaging for Chinese cabbage. The CR (clubroot resistant) Shinki DH (doubled haploid) line of Chinese cabbage carries a single dominant gene, CRb, which confers resistance to the P. brassicae races 2, 4, and 8. An F2 population derived from a cross between the CR Shinki DH line and a susceptible line, 94SK, was used to map the CRb gene. Inoculation of F3 families with SSI (single-spore isolate) resulted in a 1:2:1 segregation ratio. Use of the AFLP technique combined with bulked segregant analysis allowed five co-dominant AFLP markers, and four and seven dominant AFLP markers linked in coupling and repulsion, respectively, to be identified. Six of the 16 AFLP markers showing low frequencies of recombination with the CRb locus among 138 F2 lines were cloned. A reliable conversion procedure allowed five AFLP markers to be successfully converted into CAPS and SCAR markers. An F2 population (143 plants) was analyzed with these markers and a previously identified SCAR marker, and a genetic map around CRb covering a total distance of 6.75 cM was constructed. One dominant marker, TCR09, was located 0.78 cM from CRb. The remaining markers (TCR05, TCR01, TCR10, TCR08, and TCR03) were located on the other side of CRb, and the nearest of these was TCR05, at a distance of 1.92 cM.