MicroRNA-24-3p promotes skeletal muscle differentiation and regeneration by regulating HMGA1

Numerous studies have established the critical roles of microRNAs in regulating post-transcriptional gene expression in diverse biological processes. Here, we report on the role and mechanism of miR-24-3p in skeletal muscle differentiation and regeneration. miR-24-3p promotes myoblast differentiatio...

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Bibliographic Details
Published in:	Cellular and molecular life sciences : CMLS Vol. 79; no. 3; p. 170
Main Authors:	Dey, Paromita, Soyer, Miles A., Dey, Bijan K.
Format:	Journal Article
Language:	English
Published:	Cham Springer International Publishing 01-03-2022 Springer Nature B.V
Subjects:	Animals Biochemistry Biological activity Biomedical and Life Sciences Biomedicine Cell Biology Cell Differentiation Cell Line Cell Proliferation Differentiation Gene expression HMGA1a Protein - metabolism Inhibitor of Differentiation Proteins - metabolism Life Sciences Mice MicroRNAs MicroRNAs - metabolism miRNA Muscle Development Muscle, Skeletal - cytology Muscle, Skeletal - metabolism Muscles Musculoskeletal system Myoblasts Neonates Original Original Article Post-transcription Regeneration Skeletal muscle Stem cell transplantation Stem cells Tibialis anterior muscle Regeneration miR-24-3p Skeletal muscle stem cell Myoblast Development HMGA1 microRNA Id3 Differentiation
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Summary:	Numerous studies have established the critical roles of microRNAs in regulating post-transcriptional gene expression in diverse biological processes. Here, we report on the role and mechanism of miR-24-3p in skeletal muscle differentiation and regeneration. miR-24-3p promotes myoblast differentiation and skeletal muscle regeneration by directly targeting high mobility group AT-hook 1 (HMGA1) and regulating it and its direct downstream target, the inhibitor of differentiation 3 (ID3). miR-24-3p knockdown in neonatal mice increases PAX7-positive proliferating muscle stem cells (MuSCs) by derepressing Hmga1 and Id3 . Similarly, inhibition of miR-24-3p in the tibialis anterior muscle prevents Hmga1 and Id3 downregulation and impairs regeneration. These findings provide evidence that the miR-24-3p/HMGA1/ID3 axis is required for MuSC differentiation and skeletal muscle regeneration in vivo.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1420-682X 1420-9071
DOI:	10.1007/s00018-022-04168-7