Discrimination between infectious and non-infectious human norovirus using porcine gastric mucin

• Published in: International journal of food microbiology Vol. 155; no. 3; pp. 222 - 226
• Main Authors: Dancho, Brooke A., Chen, Haiqiang, Kingsley, David H.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 16-04-2012
• Subjects: Animals; Clinical trials; Gastric Mucins - metabolism; High pressure processing; Hot Temperature; Human norovirus; Humans; mucin; Norovirus; Norovirus - physiology; Polymerase chain reaction; Porcine mucin-conjugated magnetic beads; Pressure; Reverse Transcriptase Polymerase Chain Reaction; Swine; Thermal inactivation; U.V. radiation; Ultraviolet Rays; UV inactivation; Virus Inactivation; Porcine mucin-conjugated magnetic beads; UV inactivation; Thermal inactivation; Human norovirus; High pressure processing
• ISSN: 0168-1605; 1879-3460
• DOI: 10.1016/j.ijfoodmicro.2012.02.010

Human noroviruses (NoVs) are known to bind to human histo-blood group antigens, as well as to chemically-similar porcine gastric mucins. Here, the binding ability of NoV to porcine mucin is shown to be substantially deficient after UV, thermal, and high pressure treatments. Using qRT-PCR, ≥68% of GI.1 NoV (Norwalk strain) bound to porcine gastric mucin-conjugated magnetic beads (PGM-MBs). Application of 600-MPa high pressure treatments reduced binding of the virus to PGM-MBs by 4.7-log10, as determined by qRT-PCR, while a 300-MPa pressure treatment, reduced binding to PGM-MBs by only 0.45-log10. This is consistent with a previously reported clinical trial (Leon et al., 2011. Appl. Environ Microbiol. 77:5476–5482.) which demonstrated inactivation of 4-log10 of GI.1 NoV at 600-MPa. After thermal treatment, binding to PGM-MBs decreased when samples were heated from 0 to 80°C. Ultraviolet treatments of 0.5 and 2J/cm2 reduced observed PGM-MB binding of norovirus to 33% and negligible levels, respectively, from an initially observed 84% binding for untreated NoV. Although thermal and UV treatments are generally recognized to inactivate viruses, verification of NoV inactivation by these treatments may require volunteer studies. In total, these results suggest the loss of NoV binding to porcine mucin as a potential means to preferentially exclude non-infectious virus particles from subsequent RT-PCR detection.
►Human noroviruses (NoVs) interact with HBG antigens and porcine gastric mucins (PGMs). ►GI.1 NoV binding to PGM-magnetic beads (PGM-MBs) is lost after thermal treatments. ►UV treatments result in loss of GI.1 NoV binding to PGM-MB. ►GI.1 and GII.4 NoV binding to PGM-MBs is lost after HPP treatments. ►Binding of NoVs to PGM-MBs may exclude inactivated NoV virions from RT-PCR assays.