Helicobacter pylori vacA i region polymorphism but not babA2 status associated to gastric cancer risk in northwestern Iran

• Published in: Clinical and experimental medicine Vol. 16; no. 1; pp. 57 - 63
• Main Authors: Mottaghi, Batool, Safaralizadeh, Reza, Bonyadi, Morteza, Latifi-Navid, Saeid, Somi, Mohammad Hossein
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 01-02-2016; Springer Nature B.V
• Subjects: Adhesins, Bacterial - genetics; Adult; Aged; Aged, 80 and over; Bacteria; Bacterial Proteins - genetics; Female; Gastric cancer; Genetic Association Studies; Genetic Predisposition to Disease; Genotype & phenotype; Helicobacter Infections - ethnology; Helicobacter Infections - microbiology; Helicobacter pylori; Helicobacter pylori - genetics; Helicobacter pylori - pathogenicity; Hematology; Humans; Internal Medicine; Iran - ethnology; Male; Medicine; Medicine & Public Health; Middle Aged; Oncology; Original Article; Polymorphism; Polymorphism, Single Nucleotide; Risk factors; Stomach Neoplasms - ethnology; Stomach Neoplasms - microbiology; Iran; Gastric cancer; vacA i1; Helicobacter pylori; babA2
• ISSN: 1591-9528; 1591-8890; 1591-9528
• DOI: 10.1007/s10238-014-0327-0

Helicobacter pylori
-specific genotypes have been strongly associated with an increased risk of gastric cancer (GC). The aim of the present work was to study the associations of
H. pylori
virulence factors,
vacA i
region polymorphisms and
babA2
status with GC risk in Azerbaijan patients. The DNA extracted from gastric biopsy specimens was used to access the
babA2
and
vacA
genotypes. Overall,
babA2
was present in 85.39 % (76/89) of
H. pylori
strains: 19 out of 24 (79.16 %) strains from GC, 16 out of 17 (94.14 %) strains from peptic ulcer disease (PUD) and 41 out of 48 (85.14 %) strains from chronic gastritis. No significant association was found between
babA2
genotype and clinical outcomes (
P
 > 0.05).
i1
vacA
polymorphism was detected in 46/89 (51.68 %) strains: in 21/24 (87.5 %), 6/17 (35.29 %) and 19/48 (39.58 %) patients with GC, PUD and chronic gastritis, respectively.
i2
allele was detected in 43 (48.31 %) out of all 89 strains examined: 3 (14.28 %) of 24 strains from GC, 11 (64.71 %) of 17 from PUD, and 29 (60.42 %) of 48 strains from chronic gastritis. In this study, multiple linear regression analysis confirmed the strong association of
i1
allele with GC (partial regression correlation 0.455 ± 0.101;
P
 = 0). Results of multiple logistic regression analysis showed that
vacA i1
genotype was significantly associated with GC compared with a control group (gastritis) (odds ratio 13.142, 95 % CI 3.116–55.430;
P
 = 0). Findings from the measurement of
H. pylori babA2
and
vacA
genotypes indicate a strong correlation between the
vacA
i1
allele and GC risk in the Azerbaijan area of Iran.