Oxidant and enzymatic antioxidant status (gene expression and activity) in the brain of chickens with cold-induced pulmonary hypertension

• Published in: International journal of biometeorology Vol. 59; no. 11; pp. 1615 - 1621
• Main Authors: Hassanpour, Hossein, Khalaji-Pirbalouty, Valiallah, Nasiri, Leila, Mohebbi, Abdonnaser, Bahadoran, Shahab
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01-11-2015; Springer Nature B.V
• Subjects: Animal Physiology; Animals; Antioxidants; Biological and Medical Physics; Biophysics; Brain; Brain - metabolism; Catalase - genetics; Catalase - metabolism; Chickens; Cold Temperature; Earth and Environmental Science; Environment; Environmental Health; Enzymatic activity; Enzymes; Gene Expression; Glutathione Peroxidase - genetics; Glutathione Peroxidase - metabolism; Heart Ventricles - pathology; Hypertension; Hypertension, Pulmonary - genetics; Hypertension, Pulmonary - metabolism; Hypertension, Pulmonary - pathology; Hypertrophy, Right Ventricular; Lipid Peroxidation; Male; Meteorology; Original Paper; Oxidation; Oxidizing agents; Peroxidation; Plant Physiology; Poultry; Pulmonary hypertension; Superoxide Dismutase - genetics; Superoxide Dismutase - metabolism; Enzymatic antioxidant; Broiler chicken; Ascites; Pulmonary hypertension
• ISSN: 0020-7128; 1432-1254
• DOI: 10.1007/s00484-015-0968-z

To evaluate oxidant and antioxidant status of the brain (hindbrain, midbrain, and forebrain) in chickens with cold-induced pulmonary hypertension, the measurements of lipid peroxidation, protein oxidation, antioxidant capacity, enzymatic activity, and gene expression (for catalase, glutathione peroxidase, and superoxide dismutases) were done. There were high lipid peroxidation/protein oxidation and low antioxidant capacity in the hindbrain of cold-induced pulmonary hypertensive chickens compared to control (
P
 < 0.05). In the hypertensive chickens, superoxide dismutase activity was decreased (forebrain, midbrain, and hindbrain), while catalase activity was increased (forebrain and midbrain) (
P
 < 0.05). Glutathione peroxidase activity did not change. Relative gene expression of catalase and superoxide dismutases (1 and 2) was downregulated, while glutathione peroxidase was upregulated in the brain of the cold-induced pulmonary hypertensive chickens. Probably, these situations in the oxidant and antioxidant status of the brain especially hindbrain may change its function at cardiovascular center and sympathetic nervous system to exacerbate pulmonary hypertension.